Skip to main content Skip to navigation

Dr Corinne Smith

Job Title
Reader
Department
Life Sciences
Phone
0247 652 2461
Research Interests

Interested in studying for a PhD in my lab? See PhD Opportunities for exciting opportunity in high resolution cryo-EM of clathrin networks!

The research in my laboratory involves investigating the structure and mechanism of clathrin coated vesicle formation during clathrin-mediated endocytosis using a range of structural and biophysical techniques.

Receptor-mediated endocytosis allows a cell to choose and absorb the substances it requires. During endocytosis a clathrin-coated vesicle is formed which contains only specifically selected cargo. The clathrin coat is central to the ability of the vesicle to choose its contents and we aim, through a combination of structural and biophysical analysis to determine the factors that control its formation and disassembly

We are currently investigating how the molecular chaperone, Hsc70, and its cofactor auxilin, cause clathrin cage disassembly in vitro. We are using two approaches to investigate this. In the first we use 3D cryo-electron microscopy techniques to analyse the structure of purified clathrin cages bound to a range of proteins including Hsc70 and auxilin. In the second approach we monitor the kinetics of the process of clathrin cage disassembly using a range of biophysical techniques such as fluorescence anisotropy, stopped flow kinetics, isothermal titrating calorimetry and light scattering. We are now broadening our studies to investigate the part played by adaptor proteins and nucleotide exchange factors in the formation and disassembly of clathrin cage complexes.

Dr Smith is the Director of the Advanced Bioimaging Research Technology PlatformManager: Dr Saskia Bakker

Biography

PhD University of Bristol 1992
1992 - 1995 Post-doctoral Research Associate, University of Bristol
1995 - 1999 Research Associate, MRC Laboratory of Molecular Biology, Cambridge
1999 - 2002 Research associate and MRC Career Development Fellow, Birkbeck College, London
2002 - 2004 MRC Career Development Fellow, University of Warwick
2004 - 2007 Assistant Professor, University of Warwick
2007 - 2016 Associate Professor, University of Warwick
2016 - Now Reader, University of Warwick

  • Smith, Corinne J., 2007. Editorial. Seminars in Cell and Development Biology, Elsevier, pp. 411-411
Title Funder Award start Award end
EPSRC Centre for Doctoral Training in Molecular Analytical Science Subproject for Institution # 38018 EPSRC 22 Sep 2014 21 Mar 2023
EPSRC Centre for Doctoral Training in Molecular Analytical Science EPSRC 22 Sep 2014 21 Mar 2023
Cryo-EM proposal to The Wellcome Trust MRC 01 Jan 2017 31 Dec 2021
Structural and biophysical basis of Connexin26 channel mediated disease MRC 09 Jan 2017 08 Jan 2021
Understanding how bacteria divide: a route to new antibiotics? (Mr Souvik Naskar) Medical and Life Sciences Research Fund 01 Aug 2019 31 Jul 2020
In-depth structural characterization of the tetraspanin CD81 BBSRC 01 Apr 2016 31 Mar 2019
Defining clathrin network interactions using advanced cryo-electron microscopy Royal Society 01 Oct 2016 30 Sep 2017
A facility for 3D cellular imaging that bridges light and electron microscopy (BSRC ALERT 14 Equipment grant) BBSRC 21 Jan 2015 20 Jan 2016
BBSRC grant awarded to PI .working with co-investigator at Bristol university -sub-contract BBSRC 01 Jan 2013 31 Dec 2015
Quantitative analysis of the assembly and disassembly of clathrin cages. BBSRC 01 Jan 2013 31 Dec 2015
BBSRC ISIS:High resolution cryo-electron microscopy of clathrin cage complexes BBSRC 01 Nov 2013 30 Nov 2014
Investigating the pivotal role of clathrin light chains in vesicle transport Royal Society 01 Sep 2012 30 Aug 2013
How is vesicle uncoating achieved? MRC 01 Apr 2007 31 Mar 2010
Structure and mechanism of the twin-arginine translocation (Tat) protein transporter BBSRC 01 Apr 2004 31 Mar 2007