We are interested in the molecular basis of how flowering is controlled in plants (both Arabidopsis and crop plants), and we focus primarily on the control of lfowering by daylength (photoperiod). The Arabidopsis day neutral flowering (dnf) mutant is one of the mutants that we have isolated and characterised. We showed that the DNF gene encodes an E3 ligase that regulates the expression of the CONSTANS gene and thus flowering time.
CRISPR gene editing
We are using the revolutionary CRISPR gene-editing technology to gene-edit endogenous flowering genes in plants to enable us to manipulate thier flowering time.
We have done this in Arabidopsis, creating mutations in the endogenous FT gene which results in late flowering plants (eg. FT1_4, FT1_5 & FT1_K shown in the figure below). The FT gene in these plant lines has been modified by the CRISPR/Cas9 and has had an Adenine residue either inserted or deleted 3 nucleotides upstream of the PAM site, thus resulting in inactivation of the FT gene and late flowering of those plants.
Our group has well established links with industry, such as with Rijk Zwaan on lettuce bolting, and with Elsoms Seeds on a similar project looking at the regulation of flowering time in Rocket.