Next Generation Sequencing

Short-Read Library Preparation Service

The Genomics SRL routinely produce Illumina compatible libraries using the following kits, at a per library cost;

New England Biolabs NEBNext Ultra Express RNA
New England Biolabs NEBNext Ultra II FS DNA
16S Metagenomic Amplicon libraries

We are happy to discuss other library prep protocols on a per project basis.

Sequencing on Illumina MiSeq

The MiSeq platform provides a flexible approach to next generation sequencing;

- Longer read lengths (up to 300bp, paired-end).

- Dual-indexing supported for greater depth multiplexing of samples.

If the run lengths shown here do not suit your needs or you would like any further information or help with your NGS experiment then please contact us. There are other read lengths available.

MiSeq Specs

Number of reads per run (V3 kits) = 20 million.

Nano and micro flow cells available for smaller experiments.

Good compatiabilty for 16S amplicon work.

Read Length	Total Run Time	Approx. Output
2 x 75bp	~24hrs	3Gbp
2 x 300bp	~65hrs	12Gbp

AVITI24 Specs

Number of reads per run (approx):

High Output - 1500 million

Medium Output - 750 million

Low Output - 375 million

PE 300bp available

Read Length (examples)	Total Run Time	Approx Output
2 x 150bp (high output)	38hrs	450Gbp
2 x 75bp (high output)	24hrs	225Gbp
2 x 150bp (medium output)	38hrs	225Gbp

Sequencing on Element Biosciences AVITI24

AVITI 24 provides high quality sequencing, maintaining performance and flexibility.

AVITI24 delivers a broad range of sequencing applications, from whole genome sequencing to targeted assays.

Oxford Nanopore Technologies - P2 Solo and MinION

We can assist you with you library preparation and provide sequencing using the ONT P2 Solo or MinION platform. This is a new service and we are happy to hear more about researchers' experimental needs.

P2 Solo

MinIon

Library Prep

Sample Storage

We will endeavour to store remaining sample material or completed libraries for a period of 12 months before disposal.

Data Storage

We will endeavour to store data for a period of 12 months before deletion.