The Marianas SDC is a dual-camera spinning disk confocal from 3i (Intelligent Imaging Innovations). It provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. The high numerical aperture imaging objective and super sensitive (>95% QE) cameras make this an ideal system for gentle imaging of fast, sub-cellular events.

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Components

Objectives	100x oil, 60x oil, 40x oil, 20x air
Lasers	405 nm, 488 nm, 561nm, 640 nm
FRAP	405 nm, 488 nm, 561 nm, 640 nm + 532 nm ablation unit
Cameras	2x Photometrics 95B Prime sCMOS
Stage	motorised xyz
Incubation	37 degrees and CO2
Filters	DAPI, GFP, mCherry, Cy5, 570 nm dichroic for dual-colour
Autofocus	Hardware-based autofocus