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Marianas spinning disk confocal

The Marianas SDC is a dual-camera spinning disk confocal from 3i (Intelligent Imaging Innovations). It provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. The high numerical aperture imaging objective and super sensitive (>95% QE) cameras make this an ideal system for gentle imaging of fast, sub-cellular events.


Objectives 100x oil, 60x oil, 40x oil, 20x air
Lasers 405 nm, 488 nm, 561nm, 640 nm
FRAP 405 nm, 488 nm, 561 nm, 640 nm + 532 nm ablation unit
Cameras 2x Photometrics 95B Prime sCMOS
Stage motorised xyz
Incubation 37 degrees and CO2
Filters DAPI, GFP, mCherry, Cy5, 570 nm dichroic for dual-colour
Autofocus Hardware-based autofocus