API Deltavision Elite DV1
DV1 is a widefield deconvolution microscope from Applied Precision Instruments (API). DV1 is optimised for live-cell, multi-colour fluorescence experiments and, combined with the in-built autofocus, is particularly suited to overnight timelapse imaging of cells and sub-cellular components. The image acquisition software (softWoRx) has deconvolution functionality which can be deployed to increase contrast and resolution and improve optical sectioning.
Located in MCBB room L1.23.
Hardware details for methods reporting in publications.
Components
| Objectives | 100x oil, 60x oil, 40x oil, 20x air |
| LEDs | 405 nm, 488 nm, 561nm, 640 nm |
| FRAP | 410 nm, 532 nm |
| Cameras | Photometrics CoolSNAP HQ interline CCD |
| Stage | motorised xyz |
| Incubation | 37 degrees, CO2 |
| Filters | DAPI, CFP, FITC, GFP, YFP, TRITC, mCherry, Cy5 |
| Autofocus | Hardware-based autofocus |
