Methods Reporting
| Microscope (Make, Model) | Applied Precision, Personal DeltaVision |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Olympus, 100x, 1.40, oil, UPlanSApo Olympus, 60x, 1.42, oil, PlanApo N Olympus, 40x, 1.30, oil, UPlanFL N Olympus, 20x, 0.85, oil, UPlanSApo Olympus, 10x, 0.4, air, UPlanSApo Olympus, 4x, 0.1, air, Plan N |
| Light source(s) |
Lumencor SPECTRA X light engine Excitation wavelengths, select the one/s used: DAPI: 395/25 CFP: 440/20 GFP: 470/24 YFP: 510/25 mCherry: 575/25 CY-5: 640/30 |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Polychroics: Quad: Reflection 381-401:464-492:531-556:619-644 Transmission 409-456:500-523:564-611:652-700 GFP/mCh: Reflection 464-492:561-590 Transmission 500-553:598-617 C/YFP: Reflection 400-454:496-528 Transmission 463-487:537-550 Filter sets: DAPI: ex 387/11 em 457/50 CFP: ex 430/24 em 525/50 GFP: ex 470/40 em 525/50 YFP: ex 500/20 em 535/30 TRITC: ex 575/25 em 597/45 mCherry: ex 572/28 em 632/60 CY-5: ex 640/14 em 685/40 More filter information available on request. |
| fpbase.org channel set-up | not yet available |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Olympus IX-71 Precision Control microscope incubator Tokai Hit stage top incubator Applied Precision motorised xyz stage |
| Acquisition software and settings | softWoRx v5.5.1 |
| Camera manufacturer, model | Photometrics CoolSNAP HQ2 interline CCD |