The 3i Lattice LightSheet Microscope (LLSM) has "comparable" resolution to a confocal microscope with two significant advantages: faster imaging and considerably less photo-bleaching/photo-damage. Our microscope is based in the Centre for Mechanochemical Cell Biology and was originally Wellcome funded.

The microscope rapidly sweeps a thin sheet of light through the sample and is capable of imaging highly dynamic processes with hundreds of images a second. For example a 100-plane, 2-colour Z-stack can be taken in less than 1 second. Although usually for timelapses the speed is limited by photo-toxicity/bleaching.

The gentle imaging allows continuous recording over long time scales (hundreds to thousands of volumes).

• Resolution with deconvolution up to 230 x 230 x 370 nm (x x y x z) ^[1]. If you are prioritising spatial resolution for your live samples you may be better off using a confocal with a high NA oil objective.
• Imaging depth 10-50 µm depending on sample and required image quality
• Two cameras allowing imaging in multiple channels

Please read this overviewLink opens in a new window of what to expect from a similar LLS system prior to considering use. Image processing is supported by CAMDU.

Please acknowledge the below in your publications and presentations, in addition to the usual CAMDU acknowledgment policy.

Wellcome Trust Multi-User Equipment Grant

Award Holder: Andrew McAinsh

Award Title: Probing intra-cellular dynamics with lattice light sheet microscopy

Reference: 208384/Z/17/Z