Lattice LightSheet Microscope
The Lattice LightSheet Microscope (LLSM) has comparable resolution to a confocal microscope with two significant advantages: faster imaging and considerably less photo-bleaching/photo-damage. Originally developed by Nobel Laureate Dr Eric Betzig and now commercially available from 3i. Our microscope is Wellcome funded and is based in the Centre for Mechanochemical Cell Biology.
The microscope rapidly sweeps a thin sheet of light through the sample giving unprecedented detail of highly dynamic processes with hundreds of images a second (e.g. a 100-plane, 2-colour Z-stack in ~1 second).
The gentle imaging allows continuous recording over long time scales (hundreds to thousands of volumes).
- Resolution up to 230 x 230 x 370 nm (x x y x z) [1] with 62.5x magnification
- Imaging depth up to 50 µm
- Two cameras allowing rapid imaging in multiple channels
| Objectives | 0.71NA LWD WI (excitation), 1.1NA WI (imaging) |
| Lasers | 405 nm, 488 nm, 561nm, 642 nm |
| Cameras | 2 x Hamamatsu ORCA-Flash 4.0 v3 sCMOS |
| Incubation | flexible between 22 and 37 degrees Celsius, no CO2 |
| Filters | 561LP or 594LP dichroic with 605/64 and 510/42 emission filters |
| Sample needs |
Please read this overviewLink opens in a new window of what to expect from a similar LLS system prior to considering use. At Warwick, the following differs:
- Image processing is supported by CAMDU
- Data storage is linked to the petabyte server and is transferred nightly for internal users
- Access fees are not charged but contribution of/for consumables is required
[1] Chen, B.-C. et. al. (2014). Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution. Science (New York, N.Y.), 346(6208), 1257998.
Visitors and Collaborators
Please see our Visitor Programme page.
If you are unsure about whether your experiment (or sample) is suitable for LLSM, feel free to send a general enquiry to david dot corcoran at warwick dot ac dot uk.
Click here for a comparison of our microscopes.