Multiphoton microscopy allows allows imaging at much greater depth than standard single-photon fluorescence imaging. It is the gold-standard for imaging in thick tissues. The Olympus/Evident MPE comes with two broad-spectrum femtosecond pulsed lasers, a piezo stage, and a resonant scanner, making it ideal for imaging multiple fluorophores at high speed. It also has an additional scanner for simultaneous photomanipulation of the smaple while imaging.

The system comes with fluorescence lifetime imaging (FLIM) capabilities from PicoQuant for quantitative biosensing experiments. The MPE is fully motorised and has an incubator, making it excellent for imaging live samples, in particular embryos and tissues.

Located in the basement of IBRB in room IBRB03.

Hardware details for methods reporting in publications.

Filter diagram showing available options.

Absorption spectra of some dyes (no promises on the reliability of these papers):

https://doi.org/10.1046/j.1365-2818.2002.01074.x

https://doi.org/10.1111/jmi.12255

https://doi.org/10.3390%2Fijms232113407

https://doi.org/10.1016/j.bpj.2011.12.056

Excel table from UofRochester

See fpbase.org for other dyes and fluorescent proteins.

Booking rules for MPE.

Please acknowledge the below funding in your publications, in addition to following the usual CAMDU acknowledgment policy.

BBSRC BB/T018070/1