The SoRa is a dual-camera spinning disk confocal from Nikon. It provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. In addition, it also has an second disk (SoRa) which enables super-resolution imaging at up to twice the diffraction limit. The high numerical aperture imaging objective and super sensitive (>95% QE) cameras make this an ideal system for gentle imaging of fast, sub-cellular events.

Optical configuration.

Hardware details for methods reporting in publications.

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Components

Objectives	100x oil, 60x oil, 40x oil, 20x air
Lasers	405 nm, 488 nm, 561nm, 640 nm
FRAP	405 nm, 488 nm, 561 nm, 640 nm
Cameras	2x Photometrics 95B Prime sCMOS
Stage	motorised xyz
Incubation	37 degrees and CO2
Filters	DAPI, GFP, mCherry, Cy5, 570 nm dichroic for dual-colour
Autofocus	Hardware-based autofocus