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Nikon SoRa

The SoRa is a dual-camera spinning disk confocal from Nikon. It provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. In addition, it also has an second disk (SoRa) which enables super-resolution imaging at up to twice the diffraction limit. The high numerical aperture imaging objective and super sensitive (>95% QE) cameras make this an ideal system for gentle imaging of fast, sub-cellular events.

Optical configuration.

Hardware details for methods reporting in publications.


Components

Objectives 100x oil, 60x oil, 40x oil, 20x air
Lasers 405 nm, 488 nm, 561nm, 640 nm
FRAP 405 nm, 488 nm, 561 nm, 640 nm
Cameras 2x Photometrics 95B Prime sCMOS
Stage motorised xyz
Incubation 37 degrees and CO2
Filters DAPI, GFP, mCherry, Cy5, 570 nm dichroic for dual-colour
Autofocus Hardware-based autofocus

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