The SoRa is a dual-camera spinning disk confocal from Nikon. It provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. In addition, it also has an second disk (SoRa) which enables super-resolution imaging at up to twice the diffraction limit. The high numerical aperture imaging objective and super sensitive (>95% QE) cameras make this an ideal system for gentle imaging of fast, sub-cellular events.
Hardware details for methods reporting in publications.
|Objectives||100x oil, 60x oil, 40x oil, 20x air|
|Lasers||405 nm, 488 nm, 561nm, 640 nm|
|FRAP||405 nm, 488 nm, 561 nm, 640 nm|
|Cameras||2x Photometrics 95B Prime sCMOS|
|Incubation||37 degrees and CO2|
|Filters||DAPI, GFP, mCherry, Cy5, 570 nm dichroic for dual-colour|