Q-phase methods reporting

Microscope (Make, Model)	Telight, Q-Phase G2.5
Inverted or upright	Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)	Select the one/s used: Nikon, 60x, 1.42, oil, Plan Apo λD, WD 0.15 mm Nikon, 40x, 0.95, air, Plan λD, WD 0.11 mm Nikon, 20x, 0.8 air, Plan Apo VC, WD 0.8 mm Nikon, 10x, 0.45 air, Plan Apo λD, WD 4 mm Nikon, 4x, 0.1 air, Plan Achro , WD 30 mm
Light source(s)	Select the one/s used: Phase imaging: Telight low-coherence LED 660 nm Fluorescence imaging: Lumencor Spectra III (L)
Filters, dichroic mirrors, beam splitters	Fluorescence Imaging: Select the one/s used: DAPI / FITC / TRITC / Cy5 Dichroic: Semrock FF409/493/573/652 Excitation filters: Lumencor 390/22, 475/28, 555/28, 637/12 nm Emission filters: Semrock FF01-432/36, -515/30, -595/31, -731/137 nm CFP / YFP / mCherry Dichroic: Semrock FF459/526/596 Excitation filters: Lumencor 438/29, 511/16, 575/25 nm Emission filters: Semrock FF01-482/25, -544/24, Chroma ET632/60 nm GFP / mCherry Dichroic: Chroma 59022bs Excitation filters: Lumencor 475/28, 575/25 nm Emission filters: Chroma ET525/50m, ET632/60m nm
fpbase.org channel set-up	https://www.fpbase.org/microscope/xFg6BQb7dcjAWt83AjkryQ/Link opens in a new window
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective	5% CO₂ Okolab Digital CO2-UNIT-BL with Air pump. 37.0°C Telight microscope incubator Motorised xy stage Mad CityLabs MLC 500 um z-piezo Okolab Sample Holder for stage top incubator: GS35-M: 2x 35 mm dishes + 1 microscopic slide ; OR H201-MW for multiwell plate.
Acquisition software and settings	SophiQ versions 10.0 (till 25.06.2024); 10.1 (from 25.06.2024); 10.2 (from 20.11.2024); 10.3 (from 12/08/2025). Installation dates on our system.
Camera manufacturer, model	Phase imaging: Allied Vision Alvium 1800 U-2040m Fluorescence imaging: Tucsen Dhyana 400BSI V3