In this Open Biology paper from the Dean lab, Athina Paterou and her co-authors present a set of >100 plasmids that allows endogenous gene tagging using a diverse set of protein tags and drug resistances. This facilitates extensive protein-protein interaction studies, biochemistry, and microscopy techniques understand protein function. They perform extensive validation of these tags, identifying the best (brightest, most stable) fluorescent protein for different applications, and highlighting the effect of tandem epitope tags on protein localisation and function in expansion microscopy appraches. To meet the needs to the parasitological community, they show the plasmid series works in related parasites, such as Leishmania mexicana, and create a plasmid for tagging GPI-anchored proteins.
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