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Engineering bacteria to produce pure phage-like particles for gene delivery

ACS Synthetic Biology dec20Tridgett Matthew, Abab, Maria, Osgerby Alexander, Ramirez Garcia Robert and Jaramillo Alfonso

Natural and engineered phages have been used in many applications, but their use to deliver user-defined genetic cargoes has been hampered by contamination with replicative phage, restricting use of the technology beyond the laboratory. Here we present a method to produce transducing particles without contamination. In addition, we demonstrate the use of a helper phage-free transducing particle preparation as an antimicrobial agent. This will pave the way for the development of new phage-based technologies with greater scope than lytic phage therapy.

ACS Synthetic Biology

Wed 20 Jan 2021, 07:55 | Tags: Biotechnology