Phill Stansfeld publications
In situ structure of an intact lipopolysaccharide-bound bacterial surface layer
Andriko von Kügelgen, Haiping Tang, Gail G Hardy, Danguole Kureisaite-Ciziene, Yves V Brun, Phillip J Stansfeld, Carol V Robinson, and Tanmay A M. Bharat
Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.
A mass spectrometry-based approach to distinguish annular and specific lipid binding to membrane proteins
Jani Reddy Bolla, Robin A Corey, Cagla Sahin, Joseph Gault, Alissa Hummer, Jonathan TS Hopper, David P Lane, David Drew, Timothy M Allison, Phillip J Stansfeld, Carol V Robinson and Michael landreh