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Phill Stansfeld publications

Cell dedc19In situ structure of an intact lipopolysaccharide-bound bacterial surface layer

Andriko von Kügelgen, Haiping Tang, Gail G Hardy, Danguole Kureisaite-Ciziene, Yves V Brun, Phillip J Stansfeld, Carol V Robinson, and Tanmay A M. Bharat

Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.

Cell. December 2019

Angewandte ChemieA mass spectrometry-based approach to distinguish annular and specific lipid binding to membrane proteins

Jani Reddy Bolla, Robin A Corey, Cagla Sahin, Joseph Gault, Alissa Hummer, Jonathan TS Hopper, David P Lane, David Drew, Timothy M Allison, Phillip J Stansfeld, Carol V Robinson and Michael landreh

Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically boundlipids, and non‐specific annular interactionsis a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid binding properties, we explore the ability of detergents to compete with lipids bound in different environments. In summary, we distinguish annular from non‐annular lipids based on their exchange rates in solution.

Angewandte Chemie. Dec 2019

Wed 15 Jan 2020, 09:01