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New fungal primers reveal the diversity of Mucoromycotinian arbuscular mycorrhizal fungi and their response to nitrogen application

Mirjam Seeliger, Sally Hilton, George Muscatt, Christopher Walker, David Bass, Felipe Albornoz, Rachel J Standish, Neil D Gray, Louis Mercy, Leonidas Rempelos, Carolin Schneider, Megan H Ryan, Paul E Bilsborrow, Gary D Bending

Arbuscular mycorrhizas (AM) are the most widespread terrestrial symbiosis and are both a key determinant of plant health and a major contributor to ecosystem processes through their role in biogeochemical cycling. Until recently, it was assumed that the fungi which form AM comprise the subphylum Glomeromycotina (G-AMF), and our understanding of the diversity and ecosystem roles of AM is based almost exclusively on this group. However recent evidence shows that fungi which form the distinctive 'fine root endophyte’ (FRE) AM morphotype are members of the subphylum Mucoromycotina (M-AMF), so that AM symbioses are actually formed by two distinct groups of fungi. We investigated the influence of nitrogen (N) addition and wheat variety on the assembly of AM communities under field conditions. The results demonstrate the need to consider both G-AMF and M-AMF when investigating AM communities, and highlight the importance of primer choice when investigating AMF community dynamics.

Environmental Microbiome. September 2024

Mon 14 Oct 2024, 08:40 | Tags: Environment & Ecology