Job Title
Reader
Department
Life Sciences
Phone
0247 652 2461
Research Interests
Interested in studying for a PhD in my lab? See PhD Opportunities for exciting opportunity in high resolution cryo-EM of clathrin networks!
The research in my laboratory involves investigating the structure and mechanism of clathrin coated vesicle formation during clathrin-mediated endocytosis using a range of structural and biophysical techniques.
Receptor-mediated endocytosis allows a cell to choose and absorb the substances it requires. During endocytosis a clathrin-coated vesicle is formed which contains only specifically selected cargo. The clathrin coat is central to the ability of the vesicle to choose its contents and we aim, through a combination of structural and biophysical analysis to determine the factors that control its formation and disassembly
We are currently investigating how the molecular chaperone, Hsc70, and its cofactor auxilin, cause clathrin cage disassembly in vitro. We are using two approaches to investigate this. In the first we use 3D cryo-electron microscopy techniques to analyse the structure of purified clathrin cages bound to a range of proteins including Hsc70 and auxilin. In the second approach we monitor the kinetics of the process of clathrin cage disassembly using a range of biophysical techniques such as fluorescence anisotropy, stopped flow kinetics, isothermal titrating calorimetry and light scattering. We are now broadening our studies to investigate the part played by adaptor proteins and nucleotide exchange factors in the formation and disassembly of clathrin cage complexes.
Dr Smith is the Director of the Advanced Bioimaging Research Technology PlatformManager: Dr Saskia Bakker
Biography
PhD University of Bristol 1992
1992 - 1995 Post-doctoral Research Associate, University of Bristol
1995 - 1999 Research Associate, MRC Laboratory of Molecular Biology, Cambridge
1999 - 2002 Research associate and MRC Career Development Fellow, Birkbeck College, London
2002 - 2004 MRC Career Development Fellow, University of Warwick
2004 - 2007 Assistant Professor, University of Warwick
2007 - 2016 Associate Professor, University of Warwick
2016 - Now Reader, University of Warwick
- Møller-Olsen, Christian, Ross, Toby, Leppard, Keith, Foisor, Veronica, Smith, Corinne J., Grammatopoulos, Dimitris, Sagona, Antonia P., 2020. Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function. Scientific Reports, 10
- Turnbull, Robert E., Fairall, Louise, Saleh, Almutasem, Kelsall, Emma, Morris, Kyle L., Ragan, T. J., Savva, Christos G., Chandru, Aditya, Millard, Christopher J., Makarova, Olga V., Smith, Corinne J., Roseman, Alan M., Fry, Andrew M., Cowley, Shaun M., Schwabe, John W. R., 2020. The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure. Nature Communications, 11 (1)
- Smith, Sarah Marie, Walker, Kelly L., Jones, Alexander S., Smith, Corinne J., Robinson, Colin, 2019. Characterisation of a novel method for the production of single-span membrane proteins in Escherichia coli. Biotechnology and Bioengineering, 116 (4), pp. 722-733
- Lee, Sarah C., Collins, Richard, Lin, Yu-pin, Jamshad, Mohammed, Broughton, Claire E., Harris, Sarah A., Hanson, Benjamin S, Tognoloni, Cecilia, Parslow, Rosemary A., Terry, Ann E., Rodger, Alison, Smith, Corinne J., Edler, Karen J., Ford, Robert, Roper, David I., Dafforn, Timothy R., 2019. Nano-encapsulated escherichia coli divisome anchor ZipA, and in complex with FtsZ. Scientific Reports, 9 (1)
-
Pollock, Naomi, Rai, Megha, Simon, Kailene S., Hesketh, Sophie J., Teo, Alvin C. K., Parmar, Mayuriben, Sridhar, Pooja, Collins, Richard, Lee, Sarah C., Stroud, Zoe N., Bakker, Saskia, Muench, Stephen P., Barton, C. Howard, Hurlbut, Gregory, Roper, David I., Smith, Corinne J., Knowles, Timothy J., Spickett, Corinne M., East, J. Malcolm, Postis, Vincent et al, 2019. SMA-PAGE : a new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis. Biochimica et Biophysica Acta (BBA) - Biomembranes, 186 (8), pp. 1437-1445
- Morris, Kyle L., Jones, Joseph Reuben, Halebian, Mary, Wu, Shenping, Baker, Michael, Armache, Jean-Paul, Avila Ibarra, Amaurys, Sessions, Richard B., Cameron, Alexander, Cheng, Yifan, Smith, Corinne J., 2019. Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self assembly. Nature Structural & Molecular Biology, 26, pp. 890-898
- Smith, Sarah M., Walker, Kelly L., Jones, Alexander S., Smith, Corinne J., Robinson, Colin, 2018. Characterisation of a novel method for the production of single-span membrane proteins in Escherichia coli. Biotechnology and Bioengineering
- Dannhauser, Philip N., Camus, Stéphane M., Sakamoto, Kazuho, Sadacca, L. Amanda, Torres, Jorge A., Camus, Marine D., Briant, Kit, Vassilopoulos, Stéphane, Rothnie, Alice, Smith, Corinne J., Brodsky, Frances M., 2017. CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function. Journal of Biological Chemistry, 292, pp. 20834-20844
- Smith, Sarah Marie, Baker, Michael, Halebian, Mary, Smith, Corinne J., 2017. Weak molecular interactions in clathrin-mediated endocytosis. Frontiers in Molecular Biosciences, 4
- Smith, Sarah Marie, Yarwood, Andrew, Fleck, Roland A., Robinson, Colin, Smith, Corinne J., 2017. TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex. Biochemical Journal, 474 (9), pp. 1495-1508
- Millard, Christopher J., Varma, Niranjan, Saleh, Almutasem, Morris, Kyle L., Watson, Peter J., Bottrill, Andrew R., Fairall, Louise, Smith, Corinne J., Schwabe, John W. R., 2016. The structure of the core NuRD repression complex provides insights into its interaction with chromatin. eLife, 5, pp. 1-21
- Patel, Roshani, Vasilev, Cvetelin, Beck, Daniel, Monteferrante, Carmine G., Dijl, Jan Maarten van, Hunter, C. Neil, Smith, Corinne J., Robinson, Colin, 2014. A mutation leading to super-assembly of twin-arginine translocase (Tat) protein complexes. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1843 (9), pp. 1978-1986
- Barry, Nicolas P. E., Pitto-Barry, Anaïs, Romero-Canelón, Isolda, Tran, Johanna, Soldevila-Barreda, Joan J., Hands-Portman, Ian, Smith, Corinne J., Kirby, Nigel, Dove, Andrew P., O'Reilly, Rachel K., Sadler, P. J., 2014. Precious metal carborane polymer nanoparticles : characterisation of micellar formulations and anticancer activity. Faraday Discussions, 175, pp. 229-240
- Barry, Nicolas P. E., Pitto-Barry, Anaïs, Sánchez, Ana M., Dove, Andrew P., Procter, Richard J., Soldevila-Barreda, Joan J., Kirby, Nigel, Hands-Portman, Ian, Smith, Corinne J., O'Reilly, Rachel K., Beanland, R., Sadler, P. J., 2014. Fabrication of crystals from single metal atoms. Nature Communications, 5
- Young, Anna, Stoilova-McPhie, Svetla, Rothnie, Alice, Vallis, Yvonne, Harvey-Smith, Phillip, Ranson, Neil, Kent, Helen, Brodsky, Frances M., Pearse, Barbara M. F., Roseman, Alan, Smith, Corinne J., 2013. Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic, 14 (9), pp. 987-996
- Pacheco-Gómez, Raúl, Cheng, Xi, Hicks, Matthew R., Smith, Corinne J., Roper, David I., Addinall, Stephen G., Rodger, Alison, Dafforn, Tim, 2013. Tetramerization of ZapA is required for FtsZ bundling. Biochemical Journal, 449 (3), pp. 795-802
- Beck, Daniel, Vasisht, Nishi, Baglieri, J., Monteferrante, Carmine G., Dijl, Jan Maarten van, Robinson, Colin, Smith, Corinne J., 2013. Ultrastructural characterisation of Bacillus subtilis TatA complexes suggests they are too small to form homooligomeric translocation pores. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1833 (8), pp. 1811-1819
- Smith, Corinne J., 2012. Size doesn't matter! The importance of size variability in coated vesicles. Developmental Cell, 23 (1), pp. 9-10
- Baglieri, J., Beck, Daniel, Vasisht, Nishi, Smith, Corinne J., Robinson, Colin, 2012. Structure of TatA Paralog, TatE, suggests a structurally homogeneous form of Tat protein translocase that transports folded proteins of differing diameter. Journal of Biological Chemistry, 287 (10), pp. 7335-7344
- Baglieri, J., Beck, D., Vasisht, Nishi, Smith, Corinne J., Robinson, Colin, 2012. Structure of TatA paralog, TatE, suggests a tructurally homogeneous form of Tat protein translocase that transports folded proteins of differing diameter. Journal of Biological Chemistry, Vol.287 (No.10), pp. 7335-7344
- Turner, Daniel J., Portman, Ian, Dafforn, Tim, Rodger, Alison, Roper, David I., Smith, Corinne J., Turner, Matthew S., 2012. The Mechanics of FtsZ fibers. Biophysical Journal, Vol.102 (No.4), pp. 731-738
- Rothnie, Alice, Clarke, Anthony R., Kuzmic, Petr, Cameron, Angus, Smith, Corinne J., 2011. A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin. National Academy of Sciences. Proceedings, Vol.108 (No.17), pp. 6927-6932
- Soufi, Abdenour, Sawasdichai, Anyaporn, Shukla, Anshuman, Noy, Peter, Dafforn, Tim, Smith, Corinne J., Jayaraman, Padma-Sheela, Gaston, Kevin, 2010. DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers. Nucleic Acids Research, Vol.38 (No.21), pp. 7513-7525
- Knowles, Tim, Finka, Rachael, Smith, Corinne J., Lin, Yu-Pin, Dafforn, Tim, Overduin, Michael, 2009. Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. Journal of the American Chemical Society, Vol.131 (No.22), pp. 7484-7485
- Lee, Sarah C., Stoilova-Mcphie, Svetla, Baxter, Laura, Fülöp, Vilmos, Henderson, Janey, Rodger, Alison, Roper, David I., Scott, David J., Smith, Corinne J., Morgan, J. Alun W., 2007. Structural characterisation of the insecticidal toxin XptA1, reveals a 1.15 MDa tetramer with a cage-like structure. Journal of Molecular Biology, Vol.366 (No.5), pp. 1558-1568
- Smith, Corinne J., 2007. Editorial. Seminars in Cell and Development Biology, Elsevier, pp. 411-411
| Title | Funder | Award start | Award end |
|---|---|---|---|
| EPSRC Centre for Doctoral Training in Molecular Analytical Science Subproject for Institution # 38018 | EPSRC | 22 Sep 2014 | 21 Mar 2023 |
| EPSRC Centre for Doctoral Training in Molecular Analytical Science | EPSRC | 22 Sep 2014 | 21 Mar 2023 |
| Cryo-EM proposal to The Wellcome Trust | MRC | 01 Jan 2017 | 31 Dec 2021 |
| Structural and biophysical basis of Connexin26 channel mediated disease | MRC | 09 Jan 2017 | 08 Jan 2021 |
| Understanding how bacteria divide: a route to new antibiotics? (Mr Souvik Naskar) | Medical and Life Sciences Research Fund | 01 Aug 2019 | 31 Jul 2020 |
| In-depth structural characterization of the tetraspanin CD81 | BBSRC | 01 Apr 2016 | 31 Mar 2019 |
| Defining clathrin network interactions using advanced cryo-electron microscopy | Royal Society | 01 Oct 2016 | 30 Sep 2017 |
| A facility for 3D cellular imaging that bridges light and electron microscopy (BSRC ALERT 14 Equipment grant) | BBSRC | 21 Jan 2015 | 20 Jan 2016 |
| BBSRC grant awarded to PI .working with co-investigator at Bristol university -sub-contract | BBSRC | 01 Jan 2013 | 31 Dec 2015 |
| Quantitative analysis of the assembly and disassembly of clathrin cages. | BBSRC | 01 Jan 2013 | 31 Dec 2015 |
| BBSRC ISIS:High resolution cryo-electron microscopy of clathrin cage complexes | BBSRC | 01 Nov 2013 | 30 Nov 2014 |
| Investigating the pivotal role of clathrin light chains in vesicle transport | Royal Society | 01 Sep 2012 | 30 Aug 2013 |
| How is vesicle uncoating achieved? | MRC | 01 Apr 2007 | 31 Mar 2010 |
| Structure and mechanism of the twin-arginine translocation (Tat) protein transporter | BBSRC | 01 Apr 2004 | 31 Mar 2007 |