Job Title
Professor
Department
Life Sciences
Phone
02476528369
Web Link
Research Interests
David Roper is a former MRC Career Development Award fellow at the University of York and now Professor of Biochemistry and Structural Biology at the University Warwick. The Roper group uses structural biology techniques, principally X-ray structural determination, in combination with molecular biology and biochemical approaches, to investigate the molecular basis of microbial physiology in relation to antimicrobial resistance. Current areas of research include and bacterial cell wall (peptidoglycan) biosynthesis and antibiotic resistance signalling systems as well as targeting tRNA synthetases in bacterial pathogens. His research encompasses fundamental and translation approaches including assay development and drug discovery approach. In addition, his research group also uses a synthetic and systems biology approaches to obtain and reengineer pathway intermediates as chemical probes, substrates and inhibitors. This approach not only allows novel insight to the biology underpinning these pathways but also enables biotechnological exploration and exploitation.
My research interests are in focused in several main areas
1: Aminoacyl-tRNA synthetases
2. Bacterial Cell Wall Biosynthesis
3. Bacterial Cell Division
4: Antimicrobial Drug Discovery
Biography
BSc Biochemistry, University College Cardiff 1987
PhD Biochemistry, University of Leicester 1990
MRC Career Development Fellowship, 1996-2000
University of Warwick Lecturer in Structural Biology 2001-2006
University of Warwick Associate Professor of Structural Biology 2006-2014
University of Warwick Reader in Structural Biology 2014-2017
University of Warwick BBSRC-RSE Enterprise Fellow 2015-2016
University of Warwick Professor of Structural Biology 2017-present
- York, Anna, Lloyd, Adrian J., del Genio, Charo I., Shearer, Jonathan, Hinxman, Karen, Fritz, Konstantin, Fülöp, Vilmos, Dowson, Christopher G., Khalid, Syma, Roper, David I., 2021. Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane. Structure
- Lockey, Christine, Edwards, Richard J., Roper, David I., Dixon, Ann M., 2020. The extracellular domain of two-component system sensor kinase VanS from streptomyces coelicolor binds Vancomycin at a newly identified binding site. Scientific Reports, 10 (1)
- Catherwood, Anita C., Lloyd, Adrian J., Tod, Julie A., Chauhan, Smita, Slade, Susan E., Walkowiak, Grzegorz P., Galley, Nicola F., Punekar, Avinash S., Smart, Katie, Rea, Dean, Evans, Neil D., Chappell, Michael J., Roper, David I., Dowson, Christopher G., 2020. Substrate and stereochemical control of peptidoglycan cross-linking by transpeptidation by Escherichia coli PBP1B. Journal of the American Chemical Society, 142 (11), pp. 5034-5048
- Cook, Jonathan P., Baverstock, Tyler, McAndrew, Martin B. L., Stansfeld, Phillip J., Roper, David I., Crow, Allister, 2020. Insights into intrinsic resistance and bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain. Proceedings of the National Academy of Sciences of the United States of America, 117 (45), pp. 28355-28365
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Bryant, Jack Alfred, Morris, Faye C., Knowles, Timothy J., Maderbocus, Riyaz, Heinz, Eva, Boelter, Gabriela, Alodaini, Dema, Colyer, Adam, Wotherspoon, Peter J., Staunton, Kara A., Jeeves, Mark, Browning, Douglas F., Sevastsyanovich, Yanina R., Wells, Timothy J., Rossiter, Amanda E., Bavro, Vassiliy N., Sridhar, Pooja, Ward, Douglas G., Chong, Zhi-Soon, Goodall, Emily CA et al (Select to open full list), 2020. Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation. eLife, 9
- Massarweh, Ahmad, Bosco, Michael, Chantret, Isabelle, Léger, Thibaut, Jamal, Layla, Roper, David I., Dowson, Christopher G., Busca, Patricia, Bouhss, Ahmed, Gravier-Pelletier, Christine, Moore, Stuart E. H., 2019. Bacterial lipid II analogs : novel in vitro substrates for mammalian oligosaccharyl diphosphodolichol diphosphatase (DLODP) activities. Molecules, 24 (11)
- Cain, Ricky, Salimraj, Ramya, Punekar, Avinash S., Bellini, Dom, Fishwick, Colin W. G., Czaplewski, Lloyd, Scott, David J., Harris, Gemma, Dowson, Christopher G., Lloyd, Adrian J., Roper, David I., 2019. Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase. Journal of Medicinal Chemistry, 62 (21), pp. 9703-9717
- Lee, Sarah C., Collins, Richard, Lin, Yu-pin, Jamshad, Mohammed, Broughton, Claire E., Harris, Sarah A., Hanson, Benjamin S, Tognoloni, Cecilia, Parslow, Rosemary A., Terry, Ann E., Rodger, Alison, Smith, Corinne J., Edler, Karen J., Ford, Robert, Roper, David I., Dafforn, Timothy R., 2019. Nano-encapsulated escherichia coli divisome anchor ZipA, and in complex with FtsZ. Scientific Reports, 9 (1)
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Pollock, Naomi, Rai, Megha, Simon, Kailene S., Hesketh, Sophie J., Teo, Alvin C. K., Parmar, Mayuriben, Sridhar, Pooja, Collins, Richard, Lee, Sarah C., Stroud, Zoe N., Bakker, Saskia, Muench, Stephen P., Barton, C. Howard, Hurlbut, Gregory, Roper, David I., Smith, Corinne J., Knowles, Timothy J., Spickett, Corinne M., East, J. Malcolm, Postis, Vincent et al (Select to open full list), 2019. SMA-PAGE : a new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis. Biochimica et Biophysica Acta (BBA) - Biomembranes, 186 (8), pp. 1437-1445
- Simpson, Daniel H., Hapeshi, Alexia, Rogers, Nicola J., Brabec, Viktor, Clarkson, Guy J., Fox, David J., Hrabina, Ondrej, Kay, Gemma L., King, Andrew, Malina, Jaroslav, Millard, Andrew D., Moat, John, Roper, David I., Song, Hualong, Waterfield, Nicholas R., Scott, Peter, 2019. Metallohelices that kill Gram-negative pathogens using intracellular antimicrobial peptide pathways. Chemical Science, 10, pp. 9708-9720
- Kuru, Erkin, Radkov, Atanas, Meng, Xin, Egan, Alexander, Alvarez, Laura, Dowson, Amanda, Booher, Garrett, Breukink, Eefjan, Roper, David I., Cava, Felipe, Vollmer, Waldemar, Brun, Yves, VanNieuwenhze, Michael S., 2019. Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chemical Biology, 14 (12), pp. 2745-2756
- Punekar, Avinash S., Samsudin, Firdaus, Lloyd, Adrian J., Dowson, Christopher G., Scott, David J., Khalid, Syma, Roper, David I., 2018. The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization. The Cell Surface, 2, pp. 54-66
- Meyer, Karen, Addy, Christine, Akashi, Satoko, Roper, David I., Tame, Jeremy R. H., 2018. The crystal structure and oligomeric form of Escherichia coli L,D-carboxypeptidase A. Biochemical and Biophysical Research Communications, 499 (3), pp. 594-599
- Ábrányi-Balogh, Péter, Petri, László, Imre, Tímea, Szijj, Péter, Scarpino, Andrea, Hrast, Martina, Mitrovic, Ana, Fonovic, Ur?a Pecar, Németh, Krisztina, Barreteau, Hélène, Roper, David I., Horváti, Kata, Ferenczy, György G., Kos, Janko, Ila?, Janez, Gobec, Stanislav, Keseru, György M., 2018. A road map for prioritizing warheads for cysteine targeting covalent inhibitors. European Journal of Medicinal Chemistry, 160, pp. 94-107
- Ábrányi-Balogh, Péter, Petri, László, Imre, Tímea, Szijj, Péter, Scarpino, Andrea, Hrast, Martina, Mitrovic, Ana, Fonovic, Ur?a Pecar, Németh, Krisztina, Barreteau, Hélène, Roper, David I., Horváti, Kata, Ferenczy, György G, Kos, Janko, Ila?, Janez, Gobec, Stanislav, Keseru, György M, 2018. A road map for prioritizing warheads for cysteine targeting covalent inhibitors. European Journal of Medicinal Chemistry, 160, pp. 94-107
- Hrast, Martina, Jukic, Marko, Patin, Delphine, Tod, Julie, Dowson, Christopher G., Roper, David I., Barreteau, Hélène, Gobec, Stanislav, 2018. In silico identification, synthesis and biological evaluation of novel tetrazole inhibitors of MurB. Chemical Biology & Drug Design, 91 (6), pp. 1101-1112
- Tran, Anh T., Watson, Emma E., Pujari, Venugopal, Conroy, Trent, Dowman, Luke J., Giltrap, Andrew M., Pang, Angel, Wong, Weng Ruh, Linington, Roger G., Mahapatra, Sebabrata, Saunders, Jessica, Charman, Susan A., West, Nicholas P., Bugg, Tim, Tod, Julie, Dowson, Christopher G., Roper, David I., Crick, Dean C., Britton, Warwick J., Payne, Richard J., 2017. Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis. Nature Communications, 8
- Calvez, Philippe, Breukink, Eefjan, Roper, David I., Dib, Mélanie, Contreras-Martel, Carlos, Zapun, André, 2017. Substitutions in PBP2b from ß-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity. Journal of Biological Chemistry, 292 (7), pp. 2854-2865
- Vajs, Jure, Proud, Conor, Brozovic, Anamaria, Gazvoda, Martin, Lloyd, Adrian J., Roper, David I., Osmak, Maja, Ko?mrlj, Janez, Dowson, Christopher G., 2017. Diaryltriazenes as antibacterial agents against methicillin resistant Staphylococcus aureus (MRSA) and Mycobacterium smegmatis. European Journal of Medicinal Chemistry, 127, pp. 223-234
- Sychantha, David, Jones, Carys S., Little, Dustin J., Moynihan, Patrick J., Robinson, Howard, Galley, Nicola F., Roper, David I., Dowson, Christopher G., Howell, P. Lynne, Clarke, Anthony J., 2017. In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA). PLOS Pathogens, 13 (10)
- Tran, Anh T., Watson, Emma E., Pujari, Venugopal, Conroy, Trent, Dowman, Luke J., Giltrap, Andrew M., Pang, Angel, Wong, Weng-Ruh, Linington, Roger G., Saunders, Jessica, Charman, Susan A., West, Nicholas P., Bugg, Tim, Tod, Julie, Dowson, Christopher G., Roper, David I., Crick, Dean C., Britton, Warwick J., Payne, Richard J., 2017. Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis.. Nature Communications, 8
- Batson, Sarah, de Chiara, Cesira, Majce, Vita, Lloyd, Adrian J., Gobec, Stanislav, Rea, Dean, Fülöp, Vilmos, Thoroughgood, Christopher W., Simmons, Katie J., Dowson, Christopher G., Fishwick, Colin W. G., de Carvalho, Luiz Pedro S., Roper, David I., 2017. Inhibition of D-Ala : D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine. Nature Communications, 8 (1)
- Kaner, Rebecca A., Allison, Simon A., Faulkner, Alan D., Simpson, Daniel H., Waterfield, Nicholas R., Phillips, Roger M., Roper, David I., Shepherd, Samantha L., Scott, Peter, 2016. Anticancer metallohelices : nanomolar potency and high selectivity. Chemical Science, 7 (2), pp. 951-958
- Usha, Veeraraghavan, Lloyd, Adrian J., Roper, David I., Dowson, Christopher G., Kozlov, Guennadi, Gehring, Kalle, Chauhan, Smita, Imam, Hasan T., Blindauer, Claudia A., Besra, Gurdyal S., 2016. Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase. Scientific Reports, 6
- Broughton, Claire E., Berg, Hugo van den, Wemyss, Alan, Roper, David I., Rodger, Alison, 2016. Beyond the discovery void : new targets for antibacterial compounds. Science Progress, 99 (2), pp. 153-182
- Broughton, Claire E., Roper, David I., Berg, Hugo van den, Rodger, Alison, 2015. Bacterial cell division : experimental and theoretical approaches to the divisome. Science Progress, 98 (4), pp. 313-345
- Dow, Claire E., Berg, Hugo van den, Roper, David I., Rodger, Alison, 2015. Biological insights from a simulation model of the critical FtsZ accumulation required for prokaryotic cell division. Biochemistry, 54 (24), pp. 3803-3813
- Teo, Alvin, Roper, David I., 2015. Core steps of membrane-bound peptidoglycan biosynthesis : recent advances, insight and opportunities. Antibiotics, 4 (4), pp. 495-520
- Li, Qiong, Cheng, Wang, Morlot, Cécile, Bai, Xiao-Hui, Jiang, Yong-Liang, Wang, Wenjia, Roper, David I., Vernet, Thierry, Dong, Yu-Hui, Chen, Yuxing, Zhou, Cong-Zhao, 2015. Full-length structure of the major autolysin LytA. Acta Crystallographica Section D Biological Crystallography, 71 (6), pp. 1373-1381
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Zuegg, Johannes, Muldoon, Craig, Adamson, George, McKeveney, Declan, Thanh, Giang Le, Premraj, Rajaratnam, Becker, Bernd, Cheng, Mu, Elliott, Alysha G., Huang, Johnny X., Butler, Mark S., Bajaj, Megha, Seifert, Joachim, Singh, Latika, Galley, Nicola F., Roper, David I., Lloyd, Adrian J., Dowson, Christopher G., Cheng, Ting-Jen, Cheng, Wei-Chieh et al (Select to open full list), 2015. Carbohydrate scaffolds as glycosyltransferase inhibitors with in vivo antibacterial activity. Nature Communications, 6, pp. 1-11
- Rodolis, Maria T., Mihalyi, Agnes, O'Reilly, Amy M., Slikas, Justinas, Roper, David I., Hancock, Robert E. W., Bugg, Tim, 2014. Identification of a novel inhibition site in Translocase MraY based upon the site of interaction with Lysis Protein E from Bacteriophage ?X174. Chembiochem, 15 (9), pp. 1300-1308
- Braddick, Darren, Sandhu, Sandeep, Roper, David I., Chappell, M. J. (Michael J.), Bugg, Tim, 2014. Observation of the time-course for peptidoglycan lipid intermediate II polymerization by Staphylococcus aureus monofunctional transglycosylase. Microbiology, 160 (Part 8), pp. 1628-1636
- Faulkner, Alan D., Kaner, Rebecca A., Abdallah, Qasem M. A., Clarkson, Guy J., Fox, David J., Gurnani, Pratik, Howson, Suzanne E., Phillips, Roger M., Roper, David I., Simpson, Daniel H., Scott, Peter, 2014. Asymmetric triplex metallohelices with high and selective activity against cancer cells. Nature Chemistry, 6 (9), pp. 797-803
- Galley, Nicola F., O'Reilly, Amy M., Roper, David I., 2014. Prospects for novel inhibitors of peptidoglycan transglycosylase. Bioorganic Chemistry, 55 (100), pp. 16-26
- Paulin, Sarah, Jamshad, Mohammed, Dafforn, Tim, Garcia-Lara, Jorge, Foster, Simon J., Galley, Nicola F., Roper, David I., Rosado, Helena, Taylor, Peter W., 2014. Surfactant-free purification of membrane protein complexes from bacteria : application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. Nanotechnology, 25 (28)
- Lloyd, Adrian J., Potter, Nicola J., Fishwick, Colin W. G., Roper, David I., Dowson, Christopher G., 2013. Adenosine Tetraphosphoadenosine drives a continuous ATP-release assay for Aminoacyl-tRNA synthetases and other Adenylate-forming enzymes. ACS Chemical Biology, 8 (10), pp. 2157-2163
- Lloyd, Adrian J., Potter, Nicola J., Fishwick, Colin W. G., Roper, David I., Dowson, Christopher G., 2013. Adenosine tetraphosphoadenosine drives a continuous ATP-release assay for aminoacyl-tRNA synthetases and other adenylate-forming enzymes. ACS Chemical Biology, 8 (10), pp. 2157-2163
- Dow, Claire E., Rodger, Alison, Roper, David I., Berg, Hugo van den, 2013. A model of membrane contraction predicting initiation and completion of bacterial cell division. Integrative Biology, pp. 778-795
- Meigh, Louise, Greenhalgh, Sophie A., Rodgers, Thomas L., Cann, Martin J., Roper, David I., Dale, Nicholas, 2013. CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits. eLife, 2
- Majce, Vita, Ruane, Karen M., Gobec, Stanislav, Roper, David I., 2013. Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptideD-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 69 (number 5), pp. 503-505
- Zapun, André, Philippe, Jules, Abrahams, Katherine A., Signor, Luca, Roper, David I., Breukink, Eefjan, Vernet, Thierry, 2013. In vitro reconstitution of peptidoglycan assembly from the gram-positive pathogen streptococcus pneumoniae. ACS Chemical Biology, 8 (12), pp. 2688-2696
- Valegård, Karin, Iqbal, Aman, Kershaw, Nadia J., Ivison, David, Généreux, Catherine, Dubus, Alain, Blikstad, Cecilia, Demetriades, Marina, Hopkinson, Richard J., Lloyd, Adrian J., Roper, David I., Schofield, Christopher J., Andersson, Inger, McDonough, Michael A., 2013. Structural and mechanistic studies of theorf12 gene product from the clavulanic acid biosynthesis pathway. Acta Crystallographica Section D Biological Crystallography, 69 (8), pp. 1567-1579
- Pacheco-Gómez, Raúl, Cheng, Xi, Hicks, Matthew R., Smith, Corinne J., Roper, David I., Addinall, Stephen G., Rodger, Alison, Dafforn, Tim, 2013. Tetramerization of ZapA is required for FtsZ bundling. Biochemical Journal, 449 (3), pp. 795-802
- Ruane, Karen M., Lloyd, Adrian J., Fülöp, Vilmos, Dowson, Christopher G., Barreteau, Hélène, Boniface, Audrey, Dementin, Sébastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Gobec, Stanislav, Dessen, Andréa, Roper, David I., 2013. Specificity determinants for lysine incorporation in staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex. Journal of Biological Chemistry, 288 (46)
- Yoshida, Hisashi, Kawai, Fumihiro, Obayashi, Eiji, Akashi, Satoko, Roper, David I., Tame, Jeremy R.H., Park, Sam-Yong, 2012. Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant staphylococcus aureus in the apo and cefotaxime-bound forms. Journal of Molecular Biology, 423 (3), pp. 351-364
- Turner, Daniel J., Portman, Ian, Dafforn, Tim, Rodger, Alison, Roper, David I., Smith, Corinne J., Turner, Matthew S., 2012. The Mechanics of FtsZ fibers. Biophysical Journal, Vol.102 (No.4), pp. 731-738
- Bugg, Tim, Braddick, Darren, Dowson, Christopher G., Roper, David I., 2011. Bacterial cell wall assembly : still an attractive antibacterial target. Trends in Biotechnology, Vol.29 (No.4), pp. 167-173
- Hattersley, John G., Pérez-Velázquez, J., Chappell, M. J. (Michael J.), Bearup, Daniel James, Roper, David I., Dowson, Christopher G., Bugg, Tim, Evans, Neil D., 2011. Indistinguishability and identifiability of kinetic models for the MurC reaction in peptidoglycan biosynthesis. Computer Methods and Programs in Biomedicine, Vol.104 (No.2), pp. 70-80
- Pacheco-Gómez, Raúl, Roper, David I., Dafforn, Tim, Rodger, Alison, 2011. The pH dependence of polymerization and bundling by the essential bacterial cytoskeltal protein FtsZ. PLoS One, Vol.6 (No.6)
- Batson, Sarah, Rea, Dean, Fülöp, Vilmos, Roper, David I., 2010. Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli. Journal of Experimental Psychology: Human Perception and Performance, Vol.66 (No.4), pp. 405-408
- Kawai, Fumihiro, Clarke, Thomas B., Roper, David I., Han, Gab-Jo, Hwang, Kwang Yeon, Unzai, Satoru, Obayashi, Eiji, Park, Sam-Yong, Tame, Jeremy R. H., 2010. Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae. Journal of Molecular Biology, Vol.396 (No.3), pp. 634-645
- Sova, Matej, Cadez, Gasper, Turk, Samo, Majce, Vita, Polanc, Slovenko, Batson, Sarah, Lloyd, Adrian, Roper, David I., Fishwick, Colin W. G., Gobec, Stanislav, 2009. Design and synthesis of new hydroxyethylamines as inhibitors of D-alanyl-D-lactate ligase (VanA) and D-alanyl-D-alanine ligase (DdlB). Bioorganic & Medicinal Chemistry Letters, Vol.19 (No.5), pp. 1376-1379
- Cressina, Elena, Lloyd, Adrian J., De Pascale, Gianfranco, Mok, B. James, Caddick, Stephen, Roper, David I., Dowson, Christopher G., Bugg, Tim, 2009. Inhibition of tRNA-dependent ligase MurM from Streptococcus pneumoniae by phosphonate and sulfonamide inhibitors. Bioorganic & Medicinal Chemistry, Vol.17 (No.9), pp. 3443-3455
- Clarke, Thomas B., Kawai, Fumihiro, Park, Sam-Yong, Tame, Jeremy R. H., Dowson, Christopher G., Roper, David I., 2009. Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4. Biochemistry, Vol.48 (No.12), pp. 2675-2683
- Paradis-Bleau, Catherine, Lloyd, Adrian, Sanschagrin, Francois, Maaroufi, Halim, Clarke, Thomas B., Blewett, Ann, Dowson, Christopher G., Roper, David I., Bugg, Tim, Levesque, Roger C., 2009. Pseudomonas aeruginosa MurE amide ligase : enzyme kinetics and peptide inhibitor. Biochemical Journal, Vol.421 (No. 2), pp. 263-272
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Fuetterer, Klaus, Besra, Gurdyal S., 2009. Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis. Acta Crystallographica Section D: Biological Crystallography, Vol.65 (No.4), pp. 383-387
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Besra, Gurdyal S., 2008. Characterization of Mycobacterium tuberculosis diaminopimelic acid epimerase: paired cysteine residues are crucial for racemization. FEMS Microbiology Letters, Vol.280 (No.1), pp. 57-63
- Lloyd, Adrian J., Gilbey, Andrea M., Blewett, Anne M., De Pascale, Gianfranco, El Zoeiby, Ahmed, Levesque, Roger C., Catherwood, Anita C., Tomasz, Alexander, Bugg, Tim, Roper, David I., Dowson, Christopher G., 2008. Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan. Journal of Biological Chemistry, Vol.283 (No.10), pp. 6402-6417
- Rea, Dean, Hovington, Rebecca, Rakus, John F., Gerlt, John A., Fülöp, Vilmos, Bugg, Tim, Roper, David I., 2008. Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12. Biochemistry, Vol.47 (No.38), pp. 9955-9965
- Alderwick, Luke J., Dover, Lynn G., Veerapen, Natacha, Gurcha, Sudagar S., Kremer, Laurent, Roper, David I., Pathak, Ashish K., Reynolds, Robert C., Besra, Gurdyal S., 2008. Expression, purification and characterisation of soluble GlfT and the identification of a novel galactofuranosyltransferase Rv3782 involved in priming GlfT-mediated galactan polymerisation in Mycobacterium tuberculosis. Protein Expression and Purification, Vol.58 (No.2), pp. 332-341
- De Pascale, Gianfranco, Lloyd, Adrian J., Schouten, James A., Gilbey, Andrea M., Roper, David I., Dowson, Christopher G., Bugg, Tim, 2008. Kinetic characterization of lipid II-Ala:Alanyl-tRNA ligase (MurN) from streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates. Journal of Biological Chemistry, Vol.283 (No.50), pp. 34571-34579
- Cressina, Elena, Lloyd, Adrian J., De Pascale, Gianfranco, Roper, David I., Dowson, Christopher G., Bugg, Tim, 2007. Adenosine phosphonate inhibitors of lipid II: Alanyl tRNA ligase MurM from Streptococcus pneumoniae. Bioorganic & Medicinal Chemistry Letters, Vol.17 (No.16), pp. 4654-4656
- Lysenko, Elena S., Clarke, Thomas B., Shchepetov, Mikhail, Ratner, Adam J., Roper, David I., Dowson, Christopher G., Weiser, Jeffrey N., 2007. Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing. PL o S Pathogens, Vol.3 (No.8), pp. 1073-1081
- Lysenko, Elena S., Clarke, Thomas B., Shchepetov, Mikhail, Ratner, Adam J., Roper, David I., Dowson, Christopher G., Weiser, Jeffrey N., 2007. Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing. PLoS Pathogens, Vol.3 (No.8), pp. 1073-1081
- Lee, Sarah C., Stoilova-Mcphie, Svetla, Baxter, Laura, Fülöp, Vilmos, Henderson, Janey, Rodger, Alison, Roper, David I., Scott, David J., Smith, Corinne J., Morgan, J. Alun W., 2007. Structural characterisation of the insecticidal toxin XptA1, reveals a 1.15 MDa tetramer with a cage-like structure. Journal of Molecular Biology, Vol.366 (No.5), pp. 1558-1568
- Izumi, Atsushi, Rea, Dean, Adachi, Tomoko, Unzai, Satoru, Park, Sam-Yong, Roper, David I., Tame, Jeremy R. H., 2007. Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli. Journal of Molecular Biology, Vol.370 (No.5), pp. 899-911
- Rea, Dean, Fülöp, Vilmos, Bugg, Tim, Roper, David I., 2007. Structure and mechanism of HpcH : a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli. Journal of Molecular Biology, Vol.373 (No.4), pp. 866-876
- Kishida, H., Unzai, Satoru, Roper, David I., Lloyd, A, Park, S.Y., Tame, Jeremy R. H., 2006. Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics. Biochemistry, Vol.45 (No.3), pp. 783-792
- Adachi, Tomoko, Izumi, Atsushi, Rea, Dean, Park, Sam-Yong, Tame, Jeremy R. H., Roper, David I., 2006. Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C. Acta Crystallographica Section F-Structural Biology And Crystallization Communicati, 62 (Part 10), pp. 1010-1012
- Tötemeyer, Sabine, Sheppard, Mark, Lloyd, Adrian, Roper, David I., Dowson, Christopher G., Underhill, David, Murray, Peter, Maskell, Duncan, Bryant, Clare, 2006. IFN-gamma enhances production of nitric oxide from macrophages via a mechanism that depends on nucleotide oligomerization domain-2. Journal of Immunology, Vol.176 (No.8), pp. 4804-4810
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Lloyd, Adrian J., Besra, Gurdyal S., 2006. Use of a codon alteration strategy in a novel approach to cloning the Mycobacterium tuberculosis diaminopimelic acid epimerase. FEMS Microbiology Letters, 262 (1), pp. 39-47
- Lockey, Christine, Edwards, Richard J., Roper, David I., Dixon, Ann M., 2020. Data for The extracellular domain of two-component system sensor kinase VanS from streptomyces coelicolor binds Vancomycin at a newly identified binding site.. Department of Chemistry
- Kaner, Rebecca A., Allison, Simon A., Faulkner, Alan D., Simpson, Daniel H., Waterfield, Nicholas R., Phillips, Roger M., Roper, David I., Shepherd, Samantha L., Scott, Peter, 2015. Data for Anticancer metallohelices : nanomolar potency and high selectivity. University of Warwick. Science. Chemistry. Scott Group
| Title | Funder | Award start | Award end |
|---|---|---|---|
| MRC UK China Hubs | MRC | 01 Jan 2019 | 31 Dec 2021 |
| Maintaining cell wall integrity in Gram-Negative Bacteria (Chris Graham) | UK Research and Innovation | 01 Apr 2020 | 31 Jul 2021 |
| 19 Alert mid-range Equipment Initiative: equipment to upgrade the 600 NMR console at the NMR facility. | BBSRC | 01 Nov 2020 | 31 Mar 2021 |
| Mechanistic understanding of cell wall biosynthesis to combat antimicrobial resistance: Cell Wall AMR | MRC | 01 Jul 2015 | 28 Feb 2021 |
| Understanding how bacteria divide: a route to new antibiotics? (Mr Souvik Naskar) | Medical and Life Sciences Research Fund | 01 Aug 2019 | 31 Dec 2020 |
| BBSRC FTMA Investigating the potential to exploit the mechanism of by which tRNA-dependent amino acid synthetases activate cognate and non-cognate amino acids as a strategy for future antibiotic development (Ricky Cain) | BBSRC | 01 Aug 2018 | 31 Jul 2019 |
| Accelerate CHNUK AMR discovery: Establishing and exploiting a joint China/UK platform to enable high throughput fragment based inhibitor discovery | MRC | 01 Jul 2016 | 30 Jun 2019 |
| 17 Alert bid led by Nottingham: A new multi-wavelength analytical ultracentrifuge for the study of biomeolcular interactions | BBSRC | 01 May 2018 | 30 Apr 2019 |
| Collaborative visit to India | Academy of Medical Sciences | 04 Nov 2018 | 15 Nov 2018 |
| 5th Midlands Molecular Microbiology Meeting | Company of Biologists, The | 03 Sep 2018 | 02 Oct 2018 |
| Multi-Targetting of tRNA synthetases: A paradigm shift in combating AMR | MRC | 01 Mar 2015 | 30 Nov 2017 |
| Molecular Dissection of PASTA domains from Enterococci (Researcher Mobility Grant for Christopher Thoroughgood) | Royal Society of Chemistry | 09 Mar 2017 | 08 Jun 2017 |
| Centre for integrative theoretical and experimental analytical science for application to bacterial cell division and Quality by Design (CAS-IDP) | European Commission | 01 Mar 2013 | 28 Feb 2017 |
| Team CanUK: Novel antibacterial targets, assays, probes and opportunities in bacterial cell wall biogenesis | MRC | 01 Jan 2012 | 31 Dec 2015 |
| BBSRC Link with Astra Zeneca:Reagents and assays to exploit the final steps of peptidoglycan construction | BBSRC | 01 Aug 2013 | 31 Jul 2015 |
| The biochemical characterisation of pivotal enzymes involved in mycobacterial peptidoglycan biosynthesis Birmingham-Warwick PG grant | MRC | 01 May 2011 | 30 Apr 2014 |
| (led by UCL) BBSRC project Disruption of cytoplasmic membrane-associated functions in staphylococcus aureus by epicatechin gallate led by School of Pharmacy (with Sheffield and Birmingham) | BBSRC | 04 Apr 2011 | 03 Apr 2014 |
| Subproject for Institution # 33323 - RCHAR3009 - A new Raman instrument for polarized spectroscopy of biomacromolecular systems | EPSRC | 01 Sep 2012 | 28 Feb 2014 |
| A new Raman instrument for polarized spectroscopy of biomacromolecular systems | EPSRC | 01 Sep 2012 | 28 Feb 2014 |
| Research Agreement :Investigation into bacterial metabolism - the biochemistry of cytoplasmic synthesis of peptidoglycan precursors entiteld Impact of ligase/MreB association on inhibitor discovery. | Basilea Pharmaceutica International Ltd | 01 Aug 2013 | 31 Jan 2014 |
| Unesco- loreal Visiting Fellowship for Vita Majce | UNESCO | 01 May 2012 | 30 Apr 2013 |
| UKCan - BACWAN | MRC | 01 Aug 2009 | 30 Apr 2010 |
| UK BACWAN 2 | University of Sheffield | 01 May 2008 | 30 Apr 2010 |
| Structure and Biology of Membrane Proteins | Wellcome Trust | 01 Jan 2005 | 31 Dec 2009 |
| MAR Imaging plate x ray data collection system | Wellcome Trust | 01 Sep 2003 | 31 Aug 2008 |
| UK - BaCWAN: UK-Bacterial Cell Wall Assembly Network | MRC | 01 Apr 2006 | 31 Mar 2008 |
| Exploration and Exploitation of the Enzymology of PBP Driven Peptidoglycan Biosynthesis to Discover Novel Non-beta Lactam Antibiotics | MRC | 01 Mar 2005 | 29 Feb 2008 |
| Towards a complete structure-function description of an aromatic degradative pathway | BBSRC | 01 Feb 2005 | 31 Jan 2008 |
| Molecular Physiology and structural analysis of the innate immune system triggered by bacterial cell wall components | British Pharmacological Society | 01 Oct 2004 | 30 Sep 2007 |
| Structure, Stability & Molecular Interactions In Biological Systems | BBSRC | 01 Sep 2005 | 31 May 2006 |