Research Interests

• See our paper on tomographic docking of auxins, link from group webpage.
• Molecular recognition is at the heart of the group's activities, in particular the binding and selectivity of hormones by their receptors and transport proteins. The principal projects at present are; the kinetic characterisation of the auxin receptor TIR1; the structure and function of auxin transport proteins; and the development of biosensors for quantitating hormone concentrations in vivo.
• Many of the defining responses to auxin are mediated through the receptor protein known as TIR1. In collaboration with Dr Stefan Kepinski (University of Leeds) we are investigating the auxin-mediated kinetics of Aux/IAA transcription factor binding to the SCFTIR1 complex. Much of the work uses Biacore surface plasmon resonance (SPR) to record binding in real-time. Pharmacophoric mapping of receptors informs rational design and selection of novel auxins. This work is now extended to similar studies of auxin transport proteins.
• Biosensors combine a biological recognition domain with a physicochemical output unit. Like receptor proteins, recognition domains need to recognise analytes with appropriate sensitivity and selectivity and we are collecting suitable hormone sensor elements. In order to sample from living plants in real-time we are harnessing microtechnologies by working with the experienced neurobiology electrophysiology group of Prof Nick Dale. We have developed microsensors for cytokinins with low nanomolar sensitivity
• Tomographic docking. We have developed a new application of AutoDock Vina to examine the interactions ligands make on their way down into deep binding pockets. Our work suggests molecular filters contribute to selection long before docking occurs.

Biography

Research Projects

Publications

• Novák, Ondrej, Napier, R. (Richard), Ljung, Karin, 2017. Zooming in on plant hormone analysis : tissue- and cell-specific approaches. Annual Review of Plant Biology, 68 (1), View
• Quareshy, Mussa, Prusinska, Justyna, Li, Jun, Napier, R. (Richard), 2017. A cheminformatics review of auxins as herbicides. Journal of Experimental Botany, View
• Uzunova, Veselina V., Quareshy, Mussa, Del Genio, Charo I., Napier, R. (Richard), 2016. Tomographic docking suggests the mechanism of auxin receptor TIR1 selectivity. Open Biology, View
• Lee, Sarah C., Sundaram, Shanthy, Armitage, Lynne, Evans, John P., Hawkes, Tim, Kepinski, Stefan, Ferro, Noel, Napier, R. (Richard), 2014. Defining binding efficiency and specificity of auxins for SCFTIR1/AFB-Aux/IAA co-receptor complex formation. ACS Chemical Biology, Volume 9 (Number 3), pp. 673-682, View