Our chromatography and metabolomics facility offer a wide variety of separation and analysis options. A full work flow from compound concentration to quantification can be provided using the whole range of the equipment available; from preparative HPLC to LC-MS.

Samples can also be purified or concentrated, ready for further analysis by other techniques such as NMR.

Contact

Dr Sarah Bennett (SRL Manager)

Chris de Wolf (Chromatography Specialist)

School of Life Sciences, Gibbet Hill Road, University of Warwick, CV4 7AL

Team

Access

Our technology facilities are open to internal and external users. For more information, such as access and charging, please click on the following links:

University of Warwick users - Access

University of Warwick users - link to booking calendars

University of Warwick - access charges

External users

Fair attribution policy

Preparative HPLC

• Agilent 1290/1260 Infinity II HPLC
• Purification and preparative separation of organic compounds.
• Diode array detector (190 to 950 nm).
• Evaporative light-scattering detector (ELSD).
• Sample temperature control.
• Temperature-controlled fraction collection in either 8 ml or 10 ml vials.

Semi-Preparative HPLC

• Agilent 1260 Infinity II HPLC
• Purification and preparative separation of organic compounds.
• Diode array detector (190 to 950 nm).
• Sample temperature control. Temperature-controlled fraction collection in either 5 ml or 45 ml vials.

• Autosampler for autonomous sample runs.

Thermo Dionex ICS 5000 Ion Chromatography System

• Quantification of charged compounds.
• Anion & Cation separation.
• Eluent generation system
• Conductivity and electrochemical detector.
• Sample temperature control.

Analytical HPLC

• Agilent 1260 Infinity II HPLC
• Separation and Quantification or small organic metabolites.
• Autosampler for autonomous sample runs.

• Dual-channel UV detector (190-600 nm).
• Compatible with a wide variety of analytical-scale columns.

Genevac Ez-vac 2

• Large capacity centrifugal solvent evaporator.
• Supports 2 ml, 10 ml, 50 ml and 400 ml samples.
• Wide range of solvents accepted (except HCl and HF).
• Faster drying times using customised methods and UV lamp.

Coming Soon!

Biotage V10 Evaporator

• Rapid, single-sample centrifugal evaporator.
• 16-vial autosampler rack for automated drying
• Can concentrate or dry compounds ready for freezing.
• Can pull off a range of solvents including high boiling point solvents such as DMSO.

Thermo/Bruker Impact II LCMS

• Metabolite and lipid identification.
• Low sample volume.
• Diode array detector (190 to 950 nm).

• can be integrated into work-flow with HPLC, IC or GC samples.

Other Equipment

Agilent GC-MS (Schaefer Lab)

• Quantification and identification of volatile compounds (liquid or gas phase).

• Autosampler for autonomous sample runs.

• Compatible with a wide variety of columns.

Bio-rad Protein purification system

• Theses instruments are not within the BioSRL.

Services

• LC, MS and IC Instrument Training.
• Column and solvent selection.
• Method development.
• Application support.
• Data analysis and report writing.
• Commercial sample analysis and testing.