Metabolomics BioSRL
Our metabolomics Bio-Analytical Shared Resource Laboratory (BioSRL) offer a wide variety of separation and analysis options. A full work flow from compound concentration to quantification can be provided using the whole range of the equipment available; from preparative HPLC to LC-MS.
Samples can also be purified or concentrated, ready for further analysis by other techniques such as NMR.
Contact
Dr Sarah Bennett (Head of BioSRLs)
Dr Eleonora Silvano (Metabolomics Specialist)
School of Life Sciences, Gibbet Hill Road, University of Warwick, CV4 7AL
Access
Our technology facilities are open to internal and external users. For more information, such as access and charging, please click on the following links:
University of Warwick users - Access
University of Warwick users - link to booking calendars
Instruments and Services
Preparative HPLC
- Agilent 1290/1260 Infinity II HPLC
- Purification and preparative separation of organic compounds.
- Diode array detector (190 to 950 nm).
- Evaporative light-scattering detector (ELSD).
- Sample temperature control.
- Temperature-controlled fraction collection in either 8 ml or 10 ml vials.
Analytical HPLC
- Agilent 1260 Infinity II HPLC
- Separation and quantification of small organic metabolites
- Dual-channel UV detector (190-600nm)
- Fluorescence detector
- Autosampler for autonomous sample runs.
Ion Chromatography System
- Thermo Dionex ICS5000
- Quantification of charged compounds.
- Anion & Cation separation.
- Eluent generation system
- Conductivity and electrochemical detector.
- Sample temperature control.
- Some of our targets: metabolic products (lactate, acetate), co-factors (ATP, ADP, NAD+), phosphate, nitrate...
LC-QTOF-MS
- Thermo LC and Bruker Impact II TOF MS
- Metabolite and lipid identification.
- Low sample volume.
- Diode array detector (190 to 950 nm).
- MS can be integrated into work-flow with HPLC or IC
- Some of our analysis: targeted and semi-targeted metabolomics, quantification of osmolytes, lipid identification through LC-MS/MS...
Biotage V10 Evaporator
- Rapid, single-sample centrifugal evaporator.
- 16-vial autosampler rack for automated drying
- Can concentrate or dry compounds ready for freezing.
- Can pull off a range of solvents including high boiling point solvents such as DMSO.
- Can re-solubilise the compound into a minimal solvent volume.
Services
- LC, MS and IC Instrument Training.
- Column and solvent selection.
- Method development.
- Application support.
- Data analysis and report writing.
- Commercial sample analysis and testing.