Molecular interactions techniques selection
SPR - Biacore T200
•Most accurate measurement
•Based on analyte molecular weight (kDa)
•Complete kinetic parameters output
•Limited customisation
•Heavily sensible to buffer used
•Longer run time
MST – Nanotemper Monolith
•Reliable affinity measurement
•Good for screening protein-protein interaction
•Not limited by surface chemistry
•Relying on fluorescent labelling
•Faster learning curve
BLI – Octet R96
•Kinetic parameters output
•Based on analyte size (nm)
•Faster than SPR
•Not limited by analyte buffer
•Can be used with crude extract and sera as analyte (WHEN APPROPRIATELY DILUTED IN RUNNING BUFFER)
Which is the best platform for my experiment?
If you need further help please contact:
BioSRLs@warwick.ac.uk