API Personal Deltavision 2
DV2 is a widefield deconvolution microscope from Applied Precision Instruments (API). DV2 is optimised for live-cell, multi-colour fluorescence experiments and is particularly suited to overnight timelapse imaging of cells and sub-cellular components. The image acquisition software (softWoRx) has deconvolution functionality which can be deployed to increase contrast and resolution and improve optical sectioning.
Located in MCBB room L1.11.
Hardware details for methods reporting in publications.
Components
| Objectives | 100x oil, 60x oil, 40x oil, 20x oil, 10x air, 4x air |
| LEDs | 395 nm, 440 nm, 470 nm, 510 nm, 550 nm, 575 nm, 640 nm |
| FRAP | None |
| Cameras | Photometrics CoolSNAP HQ2 interline CCD |
| Stage | motorised xyz |
| Incubation | 37 degrees, CO2 |
| Filters | DAPI, CFP, FITC, GFP, YFP, mCherry, Cy5 |
| Autofocus | Software-based autofocus |
