Latest Publications
Single-calibration cell size measurement with flow cytometry
Philip Davies, Massimo Cavallaro, Daniel Hebenstreit
Measuring the size of individual cells in high-throughput experiments is often important in biomedical research and applications. In this paper, we demonstrate that it is possible to calibrate flowcytometry laser scatter signals with accurate measures of cell diameter from separate devices and that the calibration can be conserved upon changes in the laser settings A straightforward procedure is presented that relates the flow cytometric scatter parameters to the absolute size measurements using linear models, along with a linear transformation that converts between different instrument settings on the flow cytometer. Our method makes it possible to record on a flow cytometer a cell's size in absolute units and correlate it with other features that are recorded in parallel in the fluorescence detection channels.