Professor Ioannis Nezis

Autophagy is an evolutionarily conserved catabolic process that involves the degradation of cytoplasmic material through the lysosomal pathway. It is a cellular response in nutrient starvation but it is also responsible for the removal of aggregated proteins, damaged organelles and developmental remodeling. Dysfunction in autophagy has been implicated in an increasing number of diseases from bacterial and viral infections to cancer and more recently in neurodegeneration and other ageing-related diseases. Sequestration and degradation of cytoplasmic material by autophagy can be selective through receptor and adaptor proteins. We are using mammalian cells in vitro and the fruit fly Drosophila melanogaster, as a genetically modifiable model organism to investigate the mechanisms of autophagy and selective autophagy in the context of the physiology of the cell, the system and the living organism. We are particularly interested to study selective autophagy in the context of ageing, neurodegeneration, obesity, infections and tumorigenesis.

Research: Technical Summary

In my group we are interested in the role of autophagy in health and disease. Autophagy is an evolutionarily conserved catabolic process that involves the degradation of cytoplasmic material through the lysosomal pathway. Autophagy is a cellular response in nutrient starvation but it is also responsible for the removal of aggregated proteins, damaged organelles and developmental remodeling. Recent studies have shown that dysfunction in autophagy has been implicated in an increasing number of diseases from bacterial and viral infections to cancer and more recently in neurodegeneration and other ageing-related diseases.

Interestingly, it has been shown that induction of autophagy can increase longevity in multiple animal species. Sequestration and degradation of cytoplasmic material by autophagy can be selective through receptor and adaptor proteins. It is therefore important to identify the proteins required for recognition and targeting of the various autophagic cargo for degradation and to elucidate the role of selective autophagy in normal and pathological conditions, especially at the organismal level. We are using mammalian cells in vitro and the fruit fly Drosophila melanogaster, as a genetically modifiable model organism to investigate the mechanisms of autophagy in the context of the physiology of the cell, the system and the living organism. We have developed several molecular markers for monitoring autophagy in vivo.

The major aims of our research are:

To identify novel selective autophagy receptors and adaptors
To understand the cellular and molecular mechanisms of autophagy during ageing
To elucidate the cellular and molecular mechanisms of autophagy in diseases such as neurodegeneration, obesity, bacterial and viral infections and tumorigenesis

The long term goal of our research is to develop therapeutic strategies for these diseases.

We are studying the function of autophagy genes using a combination of RNAi mediated knock down, targeted gene expression, conditional knock out and we use a wide range of approaches, including confocal microscopy, conventional electron microscopy, immuno-gold electron microscopy, biochemical methods and bioinformatics.

In the media
Pomegranates, turmeric and red grapes: the key to long life?

Article in The Guardian covering Professor Nezis' research in autophagy and links to disease and ageing.

What is intermittent fasting and how does it work?

Article in The Times covering Professor Nezis' research in autophagy with links to fasting and wellbeing.

Intermittent fasting: what is it, how does it work and is it right for you?

Article in The Guardian covering Professor Nezis' research in autophagy with links to fasting and longevity.

Biography

2020 - : Professor of Cell Biology, School of Life Sciences, University of Warwick, UK
2018 - 2020: Reader in Cell Biology, School of Life Sciences, University of Warwick, UK
2012 - 2017: Associate Professor of Cell Biology, School of Life Sciences, University of Warwick, UK
2009-2012: Assistant Professor of Cell Biology, Department of Biological Applications & Technology, University of Ioannina, Greece
2007-2009: Post-doctoral research fellow in Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
2005-2007: Post-doctoral research fellow in Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Greece
2004-2005: Post-doctoral research fellow in Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
2002-2004: National military service, Greece
1998-2002: PhD Thesis, Cell Biology, Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Greece
1993-1997: BSc, Biology, Faculty of Biology, University of Athens, Greece

Publications

Kołodziej, Marta, Tsapras, Panagiotis, Cameron, Alexander and Nezis, Ioannis P. (2024) Transcription factor deformed wings is an Atg8a-Interacting protein that regulates autophagy. Cells, 13 (22). 1897. doi:10.3390/cells13221897 ISSN 2073-4409.

Louca, Mamas, Penman, Bridget S., Cameron, Alexander and Nezis, Ioannis P. (2024) In silico assessment of LIR motif-containing proteins binding to ATG8-family proteins. In: Nezis, I. P, (ed.) Selective Autophagy. Methods in Molecular Biology, 2845 . New York: Humana, pp. 197-201. ISBN 9781071640661

Rogov, Vladimir V., Nezis, Ioannis P., Tsapras, Panagiotis, Zhang, Hong, Dagdas, Yasin, Noda, Nobuo N., Nakatogawa, Hitoshi, Wirth, Martina, Mouilleron, Stephane, McEwan, David G., Behrends, Christian, Deretic, Vojo, Elazar, Zvulun, Tooze, Sharon A., Dikic, Ivan, Lamark, Trond and Johansen, Terje (2023) Atg8 family proteins, LIR/AIM motifs and other interaction modes. Autophagy Reports, 2 (1). 2188523 . doi:10.1080/27694127.2023.2188523 ISSN 2769-4127.

Chatzichristofi, Agathangelos, Sagris, Vasileios, Pallaris, Aristos, Eftychiou, Marios, Kalvari, Ioanna, Price, Nicholas, Theodosiou, Theodosios, Iliopoulos, Ioannis, Nezis, Ioannis P. and Promponas, Vasilis J. (2023) LIRcentral : a manually curated online database of experimentally validated functional LIR motifs. Autophagy, 19 (12). pp. 3189-3200. doi:10.1080/15548627.2023.2235851 ISSN 1554-8627.

Zhang, Yan and Nezis, Ioannis P. (2022) Label-free quantitative proteomic analysis of adult Drosophila heads. STAR Protocols, 3 (4). 101830. doi:10.1016/j.xpro.2022.101830 ISSN 2666-1667.

Jacomin, Anne-Claire, Demeter, Amanda, Gul, Lejla, Lister, Ashleigh, Lipscombe, James, Invernizzi, Rachele, Branchu, Priscilla, Macaulay, Iain, Nezis, I. P. , Kingsley, Robert A., Korcsmaros, Tamas and Hautefort, Isabelle (2022) Computational prediction and experimental validation of Salmonella Typhimurium SopE-mediated fine-tuning of autophagy in intestinal epithelial cells. Frontiers in Cellular and Infection Microbiology, 12 . 834895. doi:10.3389/fcimb.2022.834895 ISSN 2235-2988.

Lalo, Ulyana, Nezis, Ioannis P. and Pankratov, Yuriy (2022) Impact of autophagy impairment on experience- and diet-related synaptic plasticity. International Journal of Molecular Sciences, 23 (16). e9228. doi:10.3390/ijms23169228 ISSN 1422-0067.

Gohel, Raksha, Rahman, Ashrafur, Lőrincz, Peter, Nagy, Anikó, Csordás, Gábor, Zhang, Yan, Juhász, Gábor and Nezis, Ioannis P. (2022) Selective autophagy and Golgi quality control in Drosophila. Autophagy, 18 (10). pp. 2508-2509. doi:10.1080/15548627.2022.2098765 ISSN 1554-8627.

Rahman, Ashrafur, Lorincz, Peter , Gohel, Raksha, Nagy, Aniko , Csordas, Gabor, Zhang, Yan, Juhasz, Gabor and Nezis, Ioannis P. (2022) GMAP is an Atg8a-interacting protein that regulates Golgi turnover In Drosophila. Cell Reports, 39 (9). 110903. doi:10.1016/j.celrep.2022.110903 ISSN 2211-1247.

Tsapras, Panagiotis and Nezis, Ioannis P. (2022) A yeast -two-hybrid screening identifies novel Atg8a-interacting proteins in Drosophila. Autophagy, 18 (5). pp. 1211-1212. doi:10.1080/15548627.2022.2045535 ISSN 1554-8627.

Tsapras, Panagiotis, Petridi, Stavroula, Chan, Selina, Geborys, Marta, Jacomin, Anne-Claire, Sagona, Antonia P., Meier, Pascal and Nezis, Ioannis P. (2022) Selective autophagy controls innate immune response through a TAK1/TAB2/SH3PX1 axis. Cell Reports, 38 (4). 110286. doi:10.1016/j.celrep.2021.110286 ISSN 2211-1247.

Petridi, Stavroula, Jacomin, Anne-Claire, Bhujabal, Zambarla, Johansen, Terje and Nezis, Ioannis P. (2021) Exploring selective autophagy in Drosophila : methods to identify Atg8-interacting proteins. In: Methods in Cell Biology. Elsevier, pp. 13-29. ISBN 9780128244876

Klionsky, Daniel J., Abdelmohsen, Kotb, Abe, Akihisa, Abedin, Md Joynal, Abeliovich, Hagai, Acevedo Arozena, Abraham, Adachi, Hiroaki, Adams, Christopher M., Adams, Peter D, Adeli, Khosrow et al.

(2021) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17 (1). pp. 1-382. doi:10.1080/15548627.2020.1797280 ISSN 1554-8627.

Jacomin, Anne-Claire, Gohel, Raksha, Hussain, Zunoon, Varga, Agnes, Maruzs, Tamas, Eddison, Mark, Sica, Margaux, Jain, Ashish, Moffat, Kevin G., Johansen, Terje, Jenny, Andreas, Juhasz, Gabor and Nezis, Ioannis P. (2021) Degradation of arouser by endosomal microautophagy is essential for adaptation to starvation in Drosophila. Life Science Alliance, 4 (2). e202000965. doi:10.26508/lsa.202000965 ISSN 2575-1077.

Jacomin, Anne-Claire, Petridi, Stavroula, Di Monaco, Marisa and Nezis, Ioannis P. (2020) A nuclear role for Atg8-family proteins. Autophagy, 16 (9). pp. 1721-1723. doi:10.1080/15548627.2020.1794356 ISSN 1554-8627.

Jacomin, Anne-Claire, Petridi, Stavroula, Di Monaco, Marisa, Bhujabal, Zambarlal, Jain, Ashish, Mulakkal, Nitha Charles, Palara, Anthimi , Powell, Emma L., Chung, Bonita, Zampronio, Cleidiane, Jones, Alexandra M., Cameron, Alexander, Johansen, Terje and Nezis, Ioannis P. (2020) Regulation of expression of autophagy genes by Atg8a-interacting partners Sequoia, YL-1 and Sir2 in Drosophila. Cell Reports, 31 (8). 107695. doi:10.1016/j.cellrep.2020.107695 ISSN 2211-1247.

Sarkar, Sovan, Vaccaro, Maria I. and Nezis, Ioannis P. (2020) Editorial : Autophagy : from big data to physiological significance. Frontiers in Cell and Developmental Biology, 7 . 376. doi:10.3389/fcell.2019.00376 ISSN 2296-634X.

Gohel, Raksha, Kournoutis, Athanasios, Petridi, Stavroula and Nezis, Ioannis P. (2020) Molecular mechanisms of selective autophagy in Drosophila. International Review of Cell and Molecular Biology, 354 . pp. 63-105. doi:10.1016/bs.ircmb.2019.08.003 ISSN 1937-6448.

Jacomin, Anne-Claire and Nezis, Ioannis P. (2020) Selective autophagic degradation of the IKK complex in Drosophila is mediated by Kenny/IKKy to control inflammation. Molecular & Cellular Oncology, 7 (1). 1682309 . doi:10.1080/23723556.2019.1682309 ISSN 2372-3556.

Chang, Kai, Kang, Ping, Liu, Ying, Huang, Kerui, Miao, Ting, Taylor, Erika, Sagona, Antonia P., Nezis, Ioannis P., Bodmer, Rolf, Ocorr, Karen and Bai, Hua (2020) TGFB-INHB/activin signaling regulates age-dependent autophagy and cardiac health through inhibition of MTORC2 Autophagy. Autophagy, 16 (10). pp. 1807-1822. doi:10.1080/15548627.2019.1704117 ISSN 1554-8627.

Jacomin, Anne-Claire, Geraki, Kalotina, Tjendana-Tjhin, Vindy, Collingwood, Joanna F. and Nezis, Ioannis P. (2019) Impact of autophagy and ageing on iron load and ferritin in Drosophila brain. Frontiers in Cell and Developmental Biology, 7 . 142 . doi:10.3389/fcell.2019.00142 ISSN 2296-634X.

Jacomin, Anne-Claire, Petridi, Stavroula, DiMonaco, Marisa, Jain, Ashish, Bhujabal, Zambarlal, Mulakkal, Nitha Charles, Palara, Anthimi, Powell, Emma L., Chung, Bonita, Zampronio, Cleidiane G., Jones, Alexandra M., Cameron, Alexander, Johansen, Terje and Nezis, Ioannis P. (2019) Atg8a interacts with transcription factor Sequoia to control the expression of autophagy genes in Drosophila. Working Paper. Cold Spring Harbor: bioRXiv.

Sudhakar, Padhmanand, Claire-Jacomin, Anne, Hautefort, Isabelle, Samavedam, Siva, Fatemian, Koorosh, Ari, Eszter, Gul, Leila, Demeter, Amanda, Jones, Emily, Korcsmaros, Tamas and Nezis, Ioannis P. (2019) Targeted interplay between bacterial pathogens and host autophagy. Autophagy, 15 (9). pp. 1620-1633. doi:10.1080/15548627.2019.1590519 ISSN 1554-8627.

Tsapras, P., Jacomin, Anne-Claire and Nezis, Ioannis P. (2019) Assays to monitor mitophagy in Drosophila. In: Autophagy. Methods in Molecular Biology, 1880 . New York, NY: Humana Press, pp. 643-653. ISBN 9781493988723

Jacomin, Anne-Claire, Gul, Lejla, Sudhakar, Padhmanand, Korcsmaros, Tamas and Nezis, Ioannis P. (2018) What we learned from big data for autophagy research. Frontiers in Cell and Developmental Biology, 6 . 92. doi:10.3389/fcell.2018.00092 ISSN 2296-634X.

Tusco, Radu, Jacomin, Anne-Claire, Jain, Ashish, Penman, Bridget S., Bowitz Larsen, Kenneth, Johansen, Terje and Nezis, Ioannis P. (2017) Kenny mediates selective autophagic degradation of the IKK complex to control innate immune responses. Nature Communications, 8 . 1264 . doi:10.1038/s41467-017-01287-9 ISSN 2041-1723.

Jacomin, Anne-Claire, Samavedam, Siva, Charles, Hannah and Nezis, Ioannis P. (2017) iLIR@viral : a web resource for LIR motif-containing proteins in viruses. Autophagy, 13 (10). pp. 1782-1789. doi:10.1080/15548627.2017.1356978 ISSN 1554-8627.

Tsapras, Panagiotis and Nezis, Ioannis P. (2017) Caspase involvement in autophagy. Cell Death & Differentiation, 24 (8). pp. 1369-1379. doi:10.1038/cdd.2017.43 ISSN 1350-9047.

Jacomin, Anne-Claire and Nezis, Ioannis P. (2017) Assays to monitor aggrephagy in Drosophila brain. In: Turksen, K., (ed.) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, 1854 . New York, NY: Humana Press, pp. 147-157. ISBN 9781493987474

Palara, Anthimi, Sagona, Antonia P. and Nezis, Ioannis P. (2016) Preparation of Drosophila follicles for transmission electron microscopy. In: Oogenesis : Methods and Protocols. Methods in Molecular Biology, 1457 . Springer, pp. 105-110. ISBN 9781493937936

Jacomin, Anne-Claire and Nezis, Ioannis P. (2016) Using fluorescent reporters to monitor autophagy in the female germline cells in Drosophila melanogaster. In: Oogenesis : Methods and Protocols. Springer, pp. 69-78. ISBN 9781493937936

Jacomin, Anne-Claire, Samavedam, Siva, Promponas, Vasilis and Nezis, Ioannis P. (2016) iLIR database : a web resource for LIR motif-containing proteins in eukaryotes. Autophagy, 12 (10). pp. 1945-1953. doi:10.1080/15548627.2016.1207016 ISSN 1554-8627.

Tsapras, Panagiotis , Sagona, Antonia P. and Nezis, Ioannis P. (2016) Immuno-gold labeling of Drosophila follicles for transmission electron microscopy. In: Oogenesis : Methods and Protocols. Methods in Molecular Biology, 1457 . Springer, pp. 97-103. ISBN 9781493937936

Klionsky, Daniel J., Abdelmohsen, Kotb, Abe, Akihisa, Abedin, Md Joynal, Abeliovich, Hagai, Acevedo Arozena, Abraham, Adachi, Hiroaki, Adams, Christopher M., Adams, Peter D, Adeli, Khosrow et al.

(2016) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 12 (1). pp. 1-222. doi:10.1080/15548627.2015.1100356 ISSN 1554-8627.

Eikenes, Åsmund H., Malerød, Lene, Christensen, Anette Lie, Steen, Chloé B., Mathieu, Juliette, Nezis, Ioannis P., Liestøl, Knut, Huynh, Jean-René, Stenmark, H. and Haglund, Kaisa (2015) ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo. PLoS Genetics, Volume 11 (Number 1). pp. 1-7. Article number e1004904. doi:10.1371/journal.pgen.1004904 ISSN 1553-7390.

Nezis, I. P., Vaccaro, Maria I., Devenish, Rodney J. and Juhász, Gábor (2014) Autophagy in development, cell differentiation, and homeodynamics: from molecular mechanisms to diseases and pathophysiology. BioMed Research International, Volume 2014 . pp. 1-2. Article number 349623. ISSN 2314-6133 doi:10.1155/2014/349623

Mulakkal, Nitha Charles, Nagy, Péter, Takats, Szabolcs, Tusco, Radu, Juhász, Gábor and Nezis, I. P. (2014) Autophagy in Drosophila : from historical studies to current knowledge. BioMed Research International, 2014 . 273473. doi:10.1155/2014/273473 ISSN 2314-6133.

Kalvari, Ioanna, Tsompanis, Stelios, Mulakkal, Nitha Charles, Osgood, Richard, Johansen, Terje, Nezis, I. P. and Promponas, Vasilis J. (2014) iLIR : a web resource for prediction of Atg8-family interacting proteins. Autophagy, Volume 10 (Number 5). doi:10.4161/auto.28260 ISSN 1554-8627.

Sagona, Antonia P., Nezis, Ioannis P. and Stenmark, H. (Harald) (2014) Association of CHMP4B and autophagy with micronuclei : implications for cataract formation. BioMed Research International, Volume 2014 . Article number 974393. doi:10.1155/2014/974393 ISSN 2314-6133.

Eskelinen, Eeva-Liisa , Liton, Paloma B., Nezis, I. P. and Piacentini, Mauro (2013) Autophagy researchers. Autophagy, Volume 9 (Number 10). pp. 1451-1454. doi:10.4161/auto.25877 ISSN 1554-8627.

Shultz, Sebastian Wolfgang, Brech, Andreas and Nezis, I. P. (2013) Time flies : autophagy during ageing in Drosophila. In: Bailly, Yannick, (ed.) Autophagy : a double-edged sword : cell survival or death? Croatia: In-Tech. ISBN 9789535110620

Nezis, I. P. (2012) The selectivity and specificity of autophagy in drosophila. Cells, Volume 1 (Number 3). pp. 248-262. doi:10.3390/cells1030248 ISSN 2073-4409.

Klionsky, Daniel J., Abdalla, Fabio C., Abeliovich, Hagai, Abraham, Robert T., Acevedo-Arozena, Abraham, Adeli, Khosrow, Agholme, Lotta, Agnello, Maria, Agostinis, Patrizia, Aguirre-Ghiso, Julio A. et al.

(2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, Vol.8 (No.4). pp. 445-544. doi:10.4161/auto.19496 ISSN 1554-8627.

Nezis, I. P. (2012) Selective Autophagy in Drosophila. International Journal of Cell Biology, Vol. 2012 . pp. 1-9. doi:10.1155/2012/146767 ISSN 1687-8876.

Nezis, I. P. and Stenmark, H. (Harald) (2012) p62 at the Interface of Autophagy, Oxidative Stress Signaling, and Cancer. Antioxidants & Redox Signaling, Vol. 17 (No. 5). pp. 786-793. doi:10.1089/ars.2011.4394 ISSN 1523-0864.

Bartlett, Bryan J., Isakson, Pauline, Lewerenz, Jan, Sanchez, Heriberto, Kotzebue, Roxanne W., Cumming, Robert C., Harris, Greg L., Nezis, I. P., Schubert, David R., Simonsen, Anne and Finley, Kim D. (2011) Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects. Autophagy, Vol. 7 (No. 6). pp. 572-583. doi:10.4161/auto.7.6.14943 ISSN 1554-8627.

Haglund, Kaisa, Nezis, I. P. and Stenmark, H. (Harald) (2011) Structure and functions of stable intercellular bridges formed by incomplete cytokinesis during development. Communicative & Integrative Biology, Vol. 4 (No. 1). pp. 1-9. doi:10.4161/cib.4.1.13550 ISSN 1942-0889.

Sagona, Antonia P., Nezis, I. P., Bache, Kristi G., Haglund, Kaisa, Bakken, Anne Cathrine, Skotheim, Rolf I. and Stenmark, H. (Harald) (2011) A Tumor-Associated Mutation of FYVE-CENT Prevents Its Interaction with Beclin 1 and Interferes with Cytokinesis. PLoS ONE, Vol. 6 (No. 3). e17086. doi:10.1371/journal.pone.0017086 ISSN 1932-6203.

Nezis, I. P., Sagona, Antonia P., Schink, Kay Oliver and Stenmark, H. (Harald) (2010) Divide and ProsPer : the emerging role of PtdIns3P in cytokinesis. Trends in Cell Biology, Volume 20 (Number 11). pp. 642-649. doi:10.1016/j.tcb.2010.08.010 ISSN 0962-8924.

Nezis, I. P., Shravage, Bhupendra V., Sagona, Antonia P., Lamark, Trond, Bjorkoy, G., Johansen, Terje, Rusten, Tor Erik, Brech, Andreas, Baehrecke, Eric H. and Stenmark, H. (Harald) (2010) Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis. The Journal of Cell Biology, Vol. 190 (No. 4). pp. 523-531. doi:10.1083/jcb.201002035 ISSN 0021-9525.

Malagoli, Davide, Abdalla, Fabio C., Cao, Yang, Feng, Qili, Fujisaki, Kozo, Gregorc, Ales, Matsuo, Tomohide, Nezis, I. P., Papassideri, Issidora S., Sass, Miklós, Silva-Zacarin, Elaine C. M., Tettamanti, Gianluca and Umemiya-Shirafuji, Rika (2010) Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives. Autophagy, Vol. 6 (No. 5). pp. 575-588. doi:10.4161/auto.6.5.11962 ISSN 1554-8627.

Nezis, I. P., Shravage, Bhupendra V., Sagona, Antonia P., Johansen, Terje, Baehrecke, Eric H. and Stenmark, H. (Harald) (2010) Autophagy as a trigger for cell death : autophagic degradation of inhibitor of apoptosis dBruce controls DNA fragmentation during late oogenesis in Drosophila. Autophagy, Vol. 6 (No. 8). pp. 1214-1215. doi:10.4161/auto.6.8.13694 ISSN 1554-8627.

Shimokawa, Noriaki, Haglund, Kaisa, Hölter, Sabine M, Grabbe, Caroline, Kirkin, Vladimir, Koibuchi, Noriyuki, Schultz, Christian, Rozman, Jan, Hoeller, Daniela, Qiu, Chun-Hong et al.

(2010) CIN85 regulates dopamine receptor endocytosis and governs behaviour in mice. The EMBO Journal, Vol. 29 (No. 14). pp. 2421-2432. doi:10.1038/emboj.2010.120 ISSN 0261-4189.

Haglund, Kaisa, Nezis, I. P., Lemus, Dafne, Grabbe, Caroline, Wesche, Jørgen, Liestøl, Knut, Dikic, Ivan, Palmer, Ruth and Stenmark, H. (Harald) (2010) Cindr Interacts with Anillin to Control Cytokinesis in Drosophila melanogaster. Current Biology, Vol. 20 (No. 10). pp. 944-950. doi:10.1016/j.cub.2010.03.068 ISSN 09609822.

Nezis, I. P., Sagona, Antonia P., Schink, Kay Oliver and Stenmark, H. (Harald) (2010) Divide and ProsPer: The emerging role of PtdIns3P in cytokinesis. Trends in Cell Biology, Vol. 20 (No. 11). pp. 642-649. doi:10.1016/j.tcb.2010.08.010 ISSN 09628924.

Sagona, Antonia P., Nezis, I. P., Pedersen, Nina Marie, Liestøl, Knut, Poulton, John, Rusten, Tor Erik, Skotheim, Rolf I., Raiborg, Camilla and Stenmark, H. (Harald) (2010) PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody. Nature Cell Biology, Vol. 12 (No. 4). pp. 362-371. doi:10.1038/ncb2036 ISSN 1465-7392.

Vaccari, Thomas, Rusten, Tor Erik, Menut, L., Nezis, I. P., Brech, Andreas, Stenmark, H. (Harald) and Bilder, David (2009) Comparative analysis of ESCRT-I, ESCRT-II and ESCRT-III function in Drosophila by efficient isolation of ESCRT mutants. Journal of Cell Science, Volume 122 (Number 14). pp. 2413-2423. doi:10.1242/jcs.046391 ISSN 0021-9533.

Nezis, I. P., Lamark, Trond, Velentzas, Athanassios D., Rusten, Tor Erik, Bjørkøy, Geir, Johansen, Terje, Papassideri, Issidora S., Stravopodis, Dimitrios J., Margaritis, Lukas H., Stenmark, H. (Harald) and Brech, Andreas (2009) Cell death duringDrosophila melanogasterearly oogenesis is mediated through autophagy. Autophagy, Vol. 5 (No. 3). pp. 298-303. doi:10.4161/auto.5.3.7454 ISSN 1554-8627.

Nezis, I. P. and Papassideri, Issidora (2008) Chapter Thirty‐Seven Monitoring Autophagy in Insect Eggs. Methods in Enzymology, Vol. 451 . pp. 669-683. doi:10.1016/s0076-6879(08)03237-0 ISSN 00766879.

Mpakou, Vicky E., Nezis, I. P., Stravopodis, Dimitrios J., Margaritis, Lukas H. and Papassideri, Issidora S. (2008) Different modes of programmed cell death during oogenesis of the silkmoth Bombyx mori. Autophagy, Vol. 4 (No. 1). pp. 97-100. ISSN 1554-8627.

Nezis, I. P., Simonsen, Anne, Sagona, Antonia P., Finley, Kim D., Gaumer, S., Contamine, D., Rusten, Tor Erik, Stenmark, H. (Harald) and Brech, Andreas (2008) Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain. The Journal of Cell Biology, Vol. 180 (No. 6). pp. 1065-1071. doi:10.1083/jcb.200711108 ISSN 0021-9525.

Hatziantoniou, Sophia, Nezis, I. P., Margaritis, Lukas H. and Demetzos, Costas (2007) Visualisation of liposomes prepared from skin and stratum corneum lipids by transmission electron microscopy. Micron, Volume 38 (Number 8). pp. 777-781. doi:10.1016/j.micron.2007.06.005 ISSN 0968-4328.

Rusten, Tor Erik, Vaccari, Thomas, Lindmo, Karine, Rodahl, Lina M.W., Nezis, I. P., Sem-Jacobsen, Catherine, Wendler, Franz, Vincent, Jean-Paul, Brech, Andreas, Bilder, David and Stenmark, H. (Harald) (2007) ESCRTs and Fab1 regulate distinct steps of autophagy. Current Biology, Volume 17 (Number 20). pp. 1817-1825. doi:10.1016/j.cub.2007.09.032 ISSN 0960-9822.

Panagopoulos, Dimitris J., Chavdoula, Evangelia D., Nezis, I. P. and Margaritis, Lukas H. (2007) Cell death induced by GSM 900-MHz and DCS 1800-MHz mobile telephony radiation. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 626 (Number 1-2). pp. 69-78. doi:10.1016/j.mrgentox.2006.08.008 ISSN 13835718.

Velentzas, Athanassios D., Nezis, I. P., Stravopodis, Dimitrios J., Papassideri, Issidora S. and Margaritis, Lukas H. (2007) Apoptosis and autophagy function cooperatively for the efficacious execution of programmed nurse cell death during Drosophila virilis oogenesis. Autophagy, Volume 3 (Number 2). pp. 130-132. doi:10.4161/auto.3582 ISSN 1554-8627.

Velentzas, Athanassios D., Nezis, I. P., Stravopodis, Dimitrios J., Papassideri, Issidora S. and Margaritis, Lukas H. (2007) Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae). The International Journal of Developmental Biology, Volume 51 (Number 1). pp. 57-66. doi:10.1387/ijdb.062164av ISSN 0214-6282.

Nezis, I. P., Stravopodis, Dimitrios J., Margaritis, Lukas H. and Papassideri, Issidora S. (2006) Chromatin condensation of ovarian nurse and follicle cells is regulated independently from DNA fragmentation during Drosophila late oogenesis. Differentiation, Volume 74 (Number 6). pp. 293-304. doi:10.1111/j.1432-0436.2006.00076.x ISSN 0301-4681.

Nezis, I. P., Stravopodis, Dimitrios J., Margaritis, Lukas H. and Papassideri, Issidora S. (2006) Follicular atresia during Dacus oleae oogenesis. Journal of Insect Physiology, Volume 52 (Number 3). pp. 282-290. doi:10.1016/j.jinsphys.2005.11.007 ISSN 0022-1910.

Nezis, I. P., Stravopodis, Dimitrios J., Margaritis, Lukas H. and Papassideri, Issidora S. (2006) Programmed cell death of follicular epithelium during the late developmental stages of oogenesis in the fruit flies Bactrocera oleae and Ceratitis capitata (Diptera, Tephritidae) is mediated by autophagy. Development, Growth and Differentiation, Volume 48 (Number 3). pp. 189-198. doi:10.1111/j.1440-169X.2006.00856.x ISSN 0012-1592.

Velentzas, Athanassios D., Nezis, I. P., Stravopodis, Dimitrios J., Papassideri, Issidora S. and Margaritis, Lukas H. (2006) Mechanisms of programmed cell death during oogenesis in Drosophila virilis. Cell and Tissue Research, Volume 327 (Number 2). pp. 399-414. doi:10.1007/s00441-006-0298-x ISSN 0302-766X.

Nezis, I. P., Stravopodis, Dimitrios J., Margaritis, Lukas H. and Papassideri, Issidora S. (2006) Autophagy is required for the degeneration of the ovarian follicular epithelium in higher diptera. Autophagy, Volume 2 (Number 4). pp. 297-298. doi:10.4161/auto.2858 ISSN 1554-8627.

Chondrogianni, N., Tzavelas, C., Pemberton, A. J., Nezis, I. P., Rivett, A. J. and Gonos, E. S. (2005) Overexpression of proteasome 5 assembled subunit increases the amount of proteasome and confers ameliorated response to oxidative stress and higher survival rates. Journal of Biological Chemistry, Volume 280 (Number 12). pp. 11840-11850. doi:10.1074/jbc.M413007200 ISSN 0021-9258.

Nezis, I. P., Stravopodis, Dimitrios J., Papassideri, Issidora S., Stergiopoulos, Costas and Margaritis, Lukas H. (2005) Morphological irregularities and features of resistance to apoptosis in thedcp-1/pita double mutated egg chambers during Drosophila oogenesis. Cell Motility and the Cytoskeleton, Volume 60 (Number 1). pp. 14-23. doi:10.1002/cm.20043 ISSN 0886-1544.

Nezis, I. P., Modes, Vassilis, Mpakou, Vicky, Stravopodis, Dimitrios J., Papassideri, Issidora S., Mammali, Ioanna and Margaritis, Lukas H. (2003) Modes of programmed cell death during Ceratitis capitata oogenesis. Tissue and Cell , Volume 35 (Number 2). pp. 113-119. doi:10.1016/S0040-8166(03)00010-7 ISSN 0040-8166.

Nezis, I. P., Stravopodis, Dimitrios, Papassideri, Issidora, Robert-Nicoud, Michel and Margaritis, Lukas (2002) Dynamics of apoptosis in the ovarian follicle cells during the late stages of Drosophila oogenesis. Cell and Tissue Research, Volume 307 (Number 3). pp. 401-409. doi:10.1007/s00441-001-0498-3 ISSN 0302-766X.

Sideratou, Zili, Foundis, John, Tsiourvas, Dimitris, Nezis, I. P., Papadimas, Georgios and Paleos, Constantinos M. (2002) A novel dendrimeric “glue” for adhesion of phosphatidyl choline-based liposomes. Langmuir, Volume 18 (Number 13). pp. 5036-5039. doi:10.1021/la020150i ISSN 0743-7463.

Nezis, I. P., Stravopodis, Dimitrios J. , Papassideri, Issidora S., Robert-Nicoud, Michel and Margaritis, Lukas H. (2000) Stage-specific apoptotic patterns during oogenesis. European Journal of Cell Biology, Volume 79 (Number 9). pp. 610-620. doi:10.1078/0171-9335-00088 ISSN 0171-9335.

Xanthou, G., Tapinos, N. I., Polihronis, M., Nezis, I. P., Margaritis, L. H. and Moutsopoulos, H. M. (1999) CD4 cytotoxic and dendritic cells in the immunopathologic lesion of Sjögren's syndrome. Clinical & Experimental Immunology, Volume 118 (Number 1). pp. 154-163. doi:10.1046/j.1365-2249.1999.01037.x ISSN 0009-9104.

Research Projects

Title	Funder	Award start	Award end
How does membrane lipid remodelling enable intracellular survival of B. cenocepacia? - BBSRC Standard Grant - September 2022	BBSRC	01 Jan 2024	31 Dec 2026
A novel component of protein aggregates regulates ageing	BBSRC	11 Feb 2022	10 Feb 2025
A broadly accessible facility microscope to probe nanoscale cellular dynamics by combined live cell super-resolution microscopy and photomanipulation (BBSRC ALERT 2021)	BBSRC	01 Aug 2022	31 Jul 2023
What is the function of a protein that controls cytoplasmic self-eating in the nucleus?	Leverhulme Trust	01 Jul 2017	30 Sept 2020
Elucidating novel roles of selective autophagy in inflammation during ageing	BBSRC	01 Apr 2017	30 Jun 2020
Molecular and Cellular Mechanisms of Selective Autophagy and their relevance to ageing	BBSRC	24 Mar 2014	23 Apr 2017
Elucidating the function of a new selective autophagy-associated protein in the regulation of immune signalling in vivo in Drosophila	Wellcome Trust	06 Jul 2015	05 Sept 2015

Teaching / Student Supervision